脑苷肌肽对APP/PS1阿尔茨海默病模型小鼠脑内胶质纤维酸性蛋白和神经元核抗原表达的影响  

Effects of cattle encephalon glycoside and ignotin on the expression of glial fibrillary acidic protein and neuronal nuclear antigen in the brain of the APP/PS1 mouse models of Alzheimer's disease

作　　者：张莹晗 胡亚卓[2] 韩志涛[2] 高雅[3] 李瑞生 孔二艳 王小宁[2] 张中健 张红红[2] Zhang Yinghan;Hu Yazhuo;Han Zhitao;Gao Ya;Li Ruisheng;Kong Eryan;Wang Xiaoning;Zhang Zhongjian;Zhang Honghong(Institute of Psychiatry and Neuroscience.Ximriang Medical University,Xinriang 453003,China;Institute of Geriatrics,the Second Medical Center,Chinese PLA General Hospital&Chinese PLA Medical Academy.Beijing Key Laboratory of Aging and Geriatrics,National Clinical Research Center for Geriatric Disease,Beijing 100853,China;Department of Geriatrics,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Research Center for Clinical and Translational Medicine,the Fifth Medical Center,Chinese People's Liberation Army General Hospital,Beijing 100039,China)

机构地区：[1]新乡医学院精神神经医学研究院,453003 [2]解放军总医院第二医学中心老年医学研究所衰老与相关疾病研究北京市重点实验室国家老年疾病临床医学研究中心,100853 [3]河北医科大学第二医院老年病科,050000 [4]解放军总医院第五医学中心临床转化医学研究中心,100039

出　　处：《中华老年医学杂志》2020年第9期1067-1071,共5页Chinese Journal of Geriatrics

基　　金：国家自然科学基金(81771492)。

摘　　要：目的研究脑苷肌肽对APP/PS1阿尔茨海默病模型小鼠脑内胶质纤维酸性蛋白(GFAP)和神经元核抗原(NeuN)表达的影响。方法5月龄APP/PS1双转基因模型小鼠36只,数字抽签随机分为模型组(氯化钠6.6 ml·kg-1·d-1腹腔注射)、脑苷肌肽组(脑腺苷肌肽6.6 ml·kg-1·d-1腹腔注射)、多奈哌齐组(多奈呱齐2 mg·kg-1·d-1灌胃),每组12只,同月龄C57BL/6J小鼠12只为正常对照组,连续给药6周,取脑组织进行免疫组化染色检测β淀粉样蛋白(Aβ)、GFAP及NeuN的表达并定量分析。结果免疫组化染色结果显示,模型组小鼠的Aβ、GFAP表达均高于正常对照组,(0.147±0.068)%比0%、(61.750±22.020)个比(26.000±4.598)个(均P<0.05),NeuN的表达低于正常对照组,0.021±0.002比0.032±0.003(P<0.05);脑苷肌肽组及多奈哌齐组的Aβ(0.058±0.055)%和(0.057±0.045)%、GFAP表达(38.250±5.418)个和(36.130±5.963)个均低于模型组(均P<0.05),NeuN的表达0.029±0.004和0.027±0.003均高于模型组(P<0.05),脑苷肌肽组Aβ、GFAP、NeuN的表达水平与多奈哌齐组比较差异无统计学意义(均P>0.05)。结论脑苷肌肽具有多靶点的神经保护作用,该作用可能是通过降低AD小鼠的Aβ、GFAP表达水平并上调NeuN的表达实现。Objective To investigate the efcts of cattle encephalon glycoside and ignotin(CEGl)on the expression of glial fibrillary acidic protein(GF AP)and neuronal nuclear antigen(NeuN)in the brain of APP/PS1 model mice of Alzheimer's disease.Methods A total of 365-month-old APP/PS1 dual-transgenic model mice were randomly divided into 3 groups:the model group(normal saline 6.6 ml.kg-1·d-1).CEGI group(CEG16.6 ml.kg-1·d-1)and donepezil group(donepezil 2 mg·kg-1·dr1),with 12 in each group.Twelve C57BL/6J mice of the same age were used as the normal control group.All mice were given drugs for 6 weeks consecutively.Brain tissue was collected for immunohistochemical staining to detect the expression of amyloidβprotein(Aβ).GFAP and NeuN,which were then analyzed quantitatively.Results The results of immunohistochemical staining indicated that levels of Aβand GF AP were higher and levels of NeuN were lower in the model group than in the normal control group(0.147±0.068%us.0%.61.750±22.020 vus.26.000±4.598,0.021±0.002 us.0.032±0.003.P<0.05).Levels of Aβand GFAP were lower and levels of NeuN were higher in the CEGI group and the donepezil group than in the model group(0.058±0.055%us.0.057±0.045%,38.250±5.418 rs.36.130±5.963.0.029±0.004 us.0.027±0.003,P<0.05)There was no significant difference in the expression of AB.GFAP and NeuN between the CEGI:group and the donepezil group(P>0.05).Conclusions CEG1 has multi-target neuroprotective:efects via down regulating the expression of Aβand GFAP and up-regulating the expression of NeuN.

关 键 词：脑苷肌酶 阿尔茨海默病 神经胶质原纤维酸性蛋白质 神经元 

分 类 号：R749.16[医药卫生—神经病学与精神病学]