响应面法优化红花羊蹄甲花色苷微波辅助提取工艺  

Optimization of Microwave-Assisted Extraction of Anthocyanins from Flower of Bauhinia Blakeana by Response Surface Methodology

作　　者：张鹏 邓清月[1] 唐森 卢翠文 谢济运 Zhang peng;Deng qingyue;Tang sen;Lu Cuiwen;Xie Jiyun(College of Food and Biochemical Engineering Guangxi Science&Technology Normal University,Labin 546119,China;Key Laboratory for Research and Development of Characteristic Yao Medicine Resources,Guangxi Science&Technology Normal University,Laibin 546119,China)

机构地区：[1]广西科技师范学院食品与生化工程学院,广西来宾546119 [2]广西科技师范学院特色瑶药资源研究与开发重点实验室,广西来宾546119

出　　处：《包装与食品机械》2020年第4期16-22,共7页Packaging and Food Machinery

基　　金：2018-2020年广西本科高校食品科学与工程特色专业及实验实训教学基地(中心)建设项目(桂教高教[2018]52号);广西金秀桐木镇瑶医瑶药创新小镇培育建设项目(桂科高字〔2018〕299号)。

摘　　要：以红花羊蹄甲花瓣为研究材料,花色苷提取量为考察指标,利用响应面分析法对红花羊蹄甲花瓣中花色苷的微波辅助提取工艺进行优化。基于单因素试验结果,选取微波功率、微波时间、乙醇体积分数、液料比等4个因素,采用4因素3水平Box-Behnken响应面试验设计优化提取工艺。结果表明,4个因素对花色苷提取量的影响大小次序为:乙醇体积分数>微波时间>液料比>微波功率;当微波功率为600 W,微波时间86 s、乙醇体积分数为73%、液料比为31:1时,花色苷提取量最大。在此最佳工艺条件下,花色苷提取量为970.8 mg/100g(n=5,RSD=0.15%)。为从红花羊蹄甲花瓣中提取花色苷提供了一种经济可行的方法,为红花羊蹄甲植物资源开发利用提供新思路。By using the petals of Bauhinia blakeana flower as the experimental raw material,and anthocyanins extraction amount as the investigation index,the microwave-assisted extraction process of anthocyanins from the petals of Bauhinia blakeana flower was optimized by response surface methodology.Four factors such as microwave power,microwave extraction time,ethanol volume fraction and ratio of liquid to solid were selected based on the single-factor test results,four factors and three levels Box-Behnken response surface test design was used to optimize the extraction process.The results show that the order of effects of the four factors on anthocyanins extraction from big to small was:ethanol volume fraction>microwave time>liquid-material ratio>microwave power;the extraction amount of anthocyanins was the biggest when the microwave power was 600 W,microwave extraction time was 86 s,ethanol concentration was 73%,and ratio of liquid to solid was 31:1.Under the best conditions,the extraction amount of anthocyanins from petals of the flower was 970.8 mg/100g(n=5,RSD=0.15%).This study provides an economical and feasible method for extraction anthocyanins from petals of Bauhinia blakeana flower,and also provides a new idea for the development and utilization of the plant resources of Bauhinia blakeana.

关 键 词：红花羊蹄甲 响应面法 花色苷 微波辅助提取 工艺优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]