Runx2和白细胞介素-17受体C基因多态性与后纵韧带骨化症发病风险和预后的相关性  

Correlations of Runx2 and interleukin-17 receptor C polymorphisms with risk and prognosis of ossification of posterior longitudinal ligament

作　　者：刘旭[1] 王磊磊[1] 马俊杰[1] 郑君涛[1] LIU Xu;WANG Leilei;MA Junjie;ZHENG Juntao(Department of Spine,the Sixth Affiliated Hospital of Xinjiang Medical University,Urumqi 830002,China)

机构地区：[1]新疆医科大学第六附属医院脊柱三科,乌鲁木齐830002

出　　处：《中华实用诊断与治疗杂志》2020年第8期826-830,共5页Journal of Chinese Practical Diagnosis and Therapy

基　　金：新疆维吾尔自治区自然科学基金(2018D01C246)。

摘　　要：目的探讨Runx2和白细胞介素-17受体C(interleukin-17 receptor C, IL-17RC)基因多态性与后纵韧带骨化症(ossification of posterior longitudinal ligament, OPLL)发病风险和预后的关系。方法经手术治疗的OPLL患者74例为OPLL组,体检健康者74例为对照组,采用基因测序法检测2组Runx2和IL-17RC基因多态性,比较2组Runx2和IL-17RC基因不同位点基因型和等位基因分布频率;采用多因素logistic回归分析Runx2和IL-17RC基因多态性对发生OPLL的影响;术前和术后12个月,OPLL组患者采用日本骨科学会(Japanese Orthopaedic Association, JOA)评分评估颈椎功能,比较OPLL组患者术后12个月Runx2和IL-17RC基因不同基因型JOA评分改善率。结果 2组Runx2基因rs16873379、rs1321075、rs1406846基因型分布频率比较差异有统计学意义(P<0.05)。2组Runx2基因rs967588、rs16873379、rs1406846和IL-17RC基因rs199772854、rs708567等位基因分布频率比较差异有统计学意义(P<0.05)。IL-17RC基因rs199772854位点A等位基因是OPLL发生的独立保护因素(OR=0.199,95%CI:0.065~0.606,P=0.004),Runx2基因rs1406846位点A等位基因是OPLL发生的独立危险因素(OR=7.145,95%CI:2.514~20.305,P<0.001)。术后12个月,Runx2基因rs16873379位点TT基因型JOA改善率[(61.73±9.04)%]高于CC型[(52.41±6.72)%](P<0.05);rs6908650位点AA基因型JOA改善率[(66.83±7.73)%]高于GG型[(57.42±6.77)%](P<0.05);rs1406846位点TT基因型JOA改善率[(66.08±7.03)%]高于AT型[(57.19±7.53)%]和AA型[(54.19±4.45)%],AT型高于AA型(P<0.05)。IL-17RC基因rs708567位点GG基因型JOA改善率[(62.57±8.39)%]高于GA型[(58.62±7.33)%]和AA型[(54.52±6.17)%],GA型高于AA型(P<0.05)。结论 Runx2及IL-17RC基因多态性与OPLL发病风险及预后具有明显相关性。Objective To investigate the correlations of Runx2 and interleukin-17 receptor C(IL-17 RC) polymorphisms with the risk for ossification of posterior longitudinal ligament(OPLL) and the prognosis. Methods Seventy-four OPLL patients undergoing surgery(OPLL group) and 74 healthy volunteers(control group) were detected the polymorphisms of Runx2 and IL-17 RC by gene sequencing. The genotypes at different loci and allele distribution frequencies of Runx2 and IL-17 RC were compared between two groups. Multivariate logistic regression was used to analyze the influences of Runx2 and IL-17 RC polymorphisms on OPLL. The cervical function was evaluated by Japanese Orthopaedic Society(JOA) before and 12 months after surgery. JOA scores of different Runx2 and IL-17 RC genotypes were compared in OPLL group 12 months after surgery. Results There were significant differences in the distribution frequencies of Runx2 gene rs16873379, rs1321075 and rs1406846, and in the allele distribution frequencies of Runx2 gene rs967588, rs16873379 and rs1406846, as well as IL-17 RC gene rs199772854 and rs708567 between two groups(P<0.05). A allele in IL-17 RC gene rs199772854(OR=0.199, 95%CI:0.065-0.606, P=0.004) was an independent protective factor for OPLL, while A allele in Runx2 rs1406846(OR=7.145, 95%CI: 2.514-20.305, P<0.001) was an independent risk factor for OPLL. In 12 months after surgery, the JOA improvement rate of Runx2 gene rs16873379 TT genotype((61.73±9.04)%) was significantly higher than that of CC genotype((52.41±6.72)%)(P<0.05), and the JOA improvement rate of rs6908650 AA genotype((66.83±7.73)%) was significantly higher than that of GG genotype((57.42±6.77)%)(P<0.05);the JOA improvement rate of rs1406846 TT genotype((66.08±7.03)%) was significantly higher than that of AT genotype((57.19±7.53)%) and AA genotype((54.19±4.45)%), and of AT genotype than that of AA genotype(P<0.05).The JOA improvement rate of IL-17 RC gene rs708567 GG genotype((62.57±8.39)%)was significantly higher than that of GA genotype((58.62±7.33)%

关 键 词：后纵韧带骨化症 RUNX2 白细胞介素-17受体C 

分 类 号：R687.3[医药卫生—骨科学]