补肾活血中药对SD大鼠慢性高眼压模型初级视皮质磷酸化FoxO1和IKK2表达的影响  

The Effect of Bushenhuoxue on primary visual cortex’s p-FoxO1 and IKK2 in the PI3K/Akt pathway in rats model of chronic elevated intraocular pressure

作　　者：袁铭悦 李翔[2] 刘红佶 刘欢[3] 李祥玉 Yuan Mingyue;Li Xiang;Liu Hongji;Liu Huan;LI Xiangyu(Ankang Hospital of Traditional Chinese Medicine,Ankang,Shanxi,725000;Teaching Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan,610072;Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan,610075)

机构地区：[1]安康市中医医院,陕西安康725000 [2]成都中医药大学附属医院,四川610072 [3]成都中医药大学,四川成都610075

出　　处：《中医眼耳鼻喉杂志》2020年第2期61-64,共4页Journal of Chinese Ophthalmology and Otorhinolaryngology

基　　金：国家自然科学基金(81373695/H2713);成都中医大学科技发展基金(030018024);成都中医药大学杏林学者计划(NO.YXRC2019009)。

摘　　要：目的观察补肾活血中药对SD大鼠慢性高眼压(EIOP)模型初级视皮质(PVC)的影响,并对其作用机理进行初步探讨。方法采用烙闭3支上巩膜静脉法,制作大鼠慢性EIOP模型,随机分为3组:对照组、模型组、给药组。连续灌胃8周,并于8周末处死大鼠,观察其对大鼠眼压(IOP),PVC尼氏小体含量、PI3K/Akt通路上p-FoxO1及IKK2表达的影响。结果①造模后大鼠IOP明显升高,造模后8周(实验结束)IOP仍较高,补精益视片有轻度降IOP作用。②免疫组化检测3组大鼠PVC中p-FoxO1和IKK2表达:模型组>给药组>对照组。结论补肾活血中药可以保护SD大鼠慢性EIOP模型视功能:降低眼压,下调p-FoxO1和IKK2的表达。Objective To observe the effect of traditional Chinese medicine(TCM)of bushenhuoxue on primary visual cortex(PVD)changes in SD rat model of chronic elevated intraocular pressure(EIOP),and explore the mechanism of it initially.Methods By unilaterally cauterizing 3 episcleral vessels establish the rat model of chronic EIOP 30 SD rats were divided into 3 groups randomly:control group,model group and treatment group.After given bushenhuoxue of TCM,or normal saline for 8 weeks,the rats were put to death.To observe the effection of bushenhuoxue of TCM on the EIOP SD rats model’s intraocular pressure(IOP),niss body and PI3 K/Akt signal pathway’s p-FoxO1 and IKK2 in primary visual cortex.Results①The establishment of chronic EIOP model in SD rats by unilaterally cauterizing 3 episcleral vessels can increase the IOP of SD rats,and it can last at least 8 weeks(at the end of the experiment),bushenhuoxue of TCM had mild the effect to reduce IOP.②The p-FoxO1 and IKK2 on PVC which were detected by immunohistochemical method in 3 groups:The p-FoxO1 and IKK2 on PVC in the model group were higest,and th drug delivery group higher than the control group.Conclusion bushenhuoxue of TCM can protect the visual function from elevating IOP,by reducing the expression of p-FoxO1 and IKK2 in PVC.

关 键 词：补肾活血 慢性高眼压模型 视功能保护 初级视皮质 磷酸化FoxO1 IKK2 

分 类 号：R77[医药卫生—眼科]