补精益视片对大鼠慢性高眼压模型视神经PI3K/Akt通路MDM2、p53表达的影响  

Effect of bujinyishi tablet on the expression of MDM2、p53 in the PI3K/Akt pathway of the optic nerve in rat model of chronic elevated intraocular pressure

作　　者：刘欢 李翔[2] 刘红佶 袁铭悦 李祥玉 Liu Huan;Li Xiang;Liu Hongji;Yuan Mingyue;Li Xiangyu(Ziyang Hospital of TCM,Ziyang,Sichuan,641300;Hospital of Chengdu University of TCM,Chengdu,Sichuan,610072;Chengdu University of TCM,Chengdu,Sichuan,610075;Ankang Hospital of Traditional Chinese Medicine,Ankang,Shanxi,725000)

机构地区：[1]资阳市中医医院,四川资阳641300 [2]成都中医药大学附属医院,四川成都610072 [3]成都中医药大学,四川成都610075 [4]安康市中医医院,陕西安康725000

出　　处：《中医眼耳鼻喉杂志》2020年第2期72-75,共4页Journal of Chinese Ophthalmology and Otorhinolaryngology

基　　金：国家自然科学基金(编号:81373695);四川省中医药管理局科学技术研究基金(编号:2018LC008);四川省科学技术厅应用基础研究项目(编号:2018JY0658);成都中医药大学杏林学者提升计划(编号:YXRC2019009)。

摘　　要：目的观察补精益视片对SD大鼠慢性高眼压(Elevated Intraocular Pressure,EIOP)模型视神经PI3K/Akt通路MDM2、p53表达的影响,探讨其作用机理。方法30只SD大鼠随机分为对照组、模型组和给药组。采用烙闭上巩膜静脉法建立大鼠慢性EIOP模型,对照组、模型组予生理盐水灌胃,给药组予补精益视片混悬液灌胃,连续8周。记录造模前、造模后即刻、造模后8周大鼠的眼压,用免疫组化法检测视神经MDM2、p53总面积、积分光密度和平均光密度。结果(1)眼压:给药组造模后8周眼压较造模后即刻低(均P<0.05)。(2)视神经MDM2:给药组总面积(0.1625±0.026)μm^2、积分光密度(2086.70±744.326)、平均光密度(210.36±2.730)均较对照组(0.2604±0.047μm^2、3548.57±906.465、215.62±1.627)低(均P<0.05),较模型组(0.0910±0.027μm^2、1195.86±334.984、202.32±3.124)高(均P<0.05)。(3)视神经p53:给药组总面积(2.6553±0.396)μm^2、积分光密度(96419.47±38603.142)、平均光密度(208.40±4.817)均较对照组(1.9827±0.350μm^2、29393.33±9977.039、199.97±5.905)高(均P<0.05),较模型组(3.3768±0.168μm^2、345824.79±56678.048、217.81±4.378)低(均P<0.05)。结论补精益视片可能有保护SD大鼠慢性EIOP模型视神经的作用,表现为轻度降低眼压,上调MDM2表达,下调p53表达。Objective To observe effect of BuJingYiShi tablet on the expression of MDM2 and p53 in PI3K/Akt pathway of optic nerve in SD Rat Model of Chronic Elevated Intraocular Pressure,and explor its mechanism.Methods Thirty SD rats were randomly divided into three groups:control group,model group and treatment group.Establishment of chronic EIOP model in rats by cauterization of superior scleral vein.The rats in treatment group were treated with the suspension of Bujing Yishing tablet after making model,control group and model group were given physiological saline for 8 consecutive weeks.Record IOP before modeling,immediately after modeling and 8 weeks after modeling.Measured the total area,integral optical density and mean optical density of the MDM2 and p53 in optic nerve.Results(1)IOP:Compared with immediately after modeling,IOP in treatment group at 8 weeks after modeling is decreased,the difference was statistically significant(all P<0.05).(2)MDM2 expression in optic nerve as follows:Total area(0.1625±0.026)μm2,integrated optical density(2086.70±744.326)and average optical density(210.36±2.730)in treatment group were lower than control group(0.2604±0.047μm^2,3548.57±906.465,202.32±3.124,all P<0.05),which were obviously higher than model group(0.0910±0.027μm^2,1195.8±334.984,217.81±4.378,all P<0.05).Conclusion Bujinyishi tablet is possible to protect the optic nerve of the chronic EIOP model of rats.Its function as follows:Slight reduction of intraocular pressure;Increase the content of myelin sheath of optic nerve;and it may up-regulate the expression of MDM2 in PI3K/Akt pathway of optic nerve and down-regulate the expression of p53 in PI3K/Akt pathway of optic nerve.

关 键 词：青光眼 SD大鼠慢性高眼压模型 视神经 补精益视片 MDM2 p53 

分 类 号：R77[医药卫生—眼科]