叶绿体蛋白CV调节质体逆向信号的研究  

Study on chloroplast protein CV regulating plastid retrograde signaling

作　　者：唐钰莹 刘阳轩 潘婷 魏明 马欣荣[1] 汪松虎[1] TANG Yuying;LIU Yangxuan;PAN Ting;WEI Ming;MA Xinrong;WANG Songhu(Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;University of Chinese Academy of Sciences,Beijing 100049,China;College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院大学,北京100049 [3]华中农业大学生命科学技术学院,武汉430070

出　　处：《植物生理学报》2020年第7期1553-1560,共8页Plant Physiology Journal

基　　金：国家自然科学基金项目(31871354)。

摘　　要：叶绿体在发育和环境适应过程中受到细胞核基因的调控,同时叶绿体也会通过某些信号分子调控细胞核基因的表达,这些来自叶绿体的信号被称为质体的逆向信号。定位于叶绿体的叶绿体囊泡化蛋白(chloroplast vesiculation,CV)在衰老或受到非生物胁迫时会诱导叶绿体形成囊泡,从而介导叶绿体蛋白的降解,而CV蛋白是否参与质体的逆向信号目前还不清楚。本实验以CV基因沉默型(amiR-CV)拟南芥(Arabidopsis thaliana)转基因植株为材料,研究植物对高光胁迫的应答,并利用qRT-PCR的方法,检测高光处理后不同逆向信号途径来源的核编码光合作用相关基因(PhANGs)的表达差异。结果表明,高光胁迫下,CV基因沉默型植株比野生型植株的存活率更高,同时DREB2A、RD29A、ZAT10及ELIP2等逆境胁迫响应基因的上调程度均显著高于野生型。说明在CV基因沉默型植株中质体逆向信号得到了增强,响应逆境的胁迫基因表达量增加,从而提高了植株对高光胁迫的耐受力,证明了CV除了能够诱导胁迫条件下叶绿体的降解,也可以通过调节质体的逆向信号来影响植物对非生物胁迫的应答。Chloroplasts are regulated by nuclear genes during development and environmental adaptation.Meanwhile,chloroplasts also regulate nuclear gene expression through certain signal molecules which are called plastid retrograde signaling.It has been reported that the chloroplast-localized protein CV mediates degradation of chloroplast proteins through inducing the formation of chloroplast vesicles when aging or under abiotic stress.However,it is unclear whether CV proteins are involved in the retrograde signaling.In this experiment,we use the transgenic CV-silenced Arabidopsis thaliana(amiR-CV)as materials to investigate the plant response to high light stress.The qRT-PCR analysis was used to detect the differential expression of nuclear-coded photosynthesis-related genes(PhANGs)from different retrograde signaling pathway after high light treatment.Our results showed that under high light stress,CV-silenced plants had higher survival rates than wild-type plants and showed the enhanced expression of stress-responsive genes,such as DREB2 A,RD29 A,ZAT10 and ELIP2,which are regulated by plastid retrograde signaling.The chloroplast retrograde signaling was enhanced in CV-silenced plants,which might increase the plant’s tolerance to high light stress by increasing the expression of stress-responsive genes.Our results showed that in addition to mediating chloroplast degradation under stress conditions,CV can also influence plant responses to abiotic stress by regulating the retrograde signaling.

关 键 词：叶绿体 逆向信号 高光胁迫 叶绿体蛋白CV 

分 类 号：Q943.2[生物学—植物学]