白及多糖超声提取工艺及其抗氧化活性研究  

Study on Ultrasonic Extraction Technology and Antioxidant Activity of Bletillastriata Polysaccharides Extraction Process

作　　者：吴诗惠[1] 王剑波 开拓 朱宁[1] 杨军[1] 季绍聪 张美[1] 杨安东[1] WU Shihui;WANG Jianbo;KAI Tuo;ZHU Ning;YANG Jun;JI Shaochong;ZHANG Mei;YANG Andong(Sichuan Academy of Chinese Medicine Sciences,Sichuan Center for Translational Medicine of Traditional Chinese Medicine, Sichuan Engineering Technology Research Center of Genuine Regional Drug, Engineering Research Center for Formation Principle and Quality Evaluation of Genuine Medicinal Materials in Sichuan Province, Biological Assay Key Laboratory of National Administration of Traditional Chinese Medicine for Traditional Chinese Medicine Quality, Chengdu 610041, China)

机构地区：[1]四川省中医药科学院,四川省中医药转化医学中心,四川省道地药材系统开发工程技术研究中心,四川省道地药材形成原理与品质评价工程研究中心,国家中医药管理局中药质量生物评价重点研究室,成都610041

出　　处：《世界中医药》2020年第17期2556-2560,共5页World Chinese Medicine

基　　金：四川省省级科研院所基本科研业务费项目(A-2013N-20)。

摘　　要：目的:优化白及多糖的超声提取工艺,比较不同产地白及多糖含量差异,考察白及多糖稳定性和抗氧化活性。方法:以多糖得率为考察指标,料液比、超声温度、超声时间为考察因素设计L9(34)正交试验优化白及多糖超声波提取工艺;以苯酚-硫酸法测定白及多糖含量,考察陕西汉中、云南普洱、湖南洪江及四川绵阳白及多糖含量产地差异;以化学方法考察白及多糖稳定性,并比较白及多糖对1,1-二苯基-2-苦肼基自由基(DPPH·)和羟基自由基(·OH)的清除率以评价其体外抗氧化活性。结果:最佳超声提取工艺条件为:料液比1∶25(g/mL)、超声温度80℃、超声时间10 min;四川绵阳白及多糖含量最高,达到60.81%,湖南洪江次之,云南普洱最低;白及多糖在柠檬酸及中性溶液中的稳定性较好,在苯甲酸钠、过酸性或过碱性溶液中的稳定性较差;白及多糖能有效地清除DPPH和羟基自由基,具有潜在的体外抗氧化活性。结论:白及多糖超声波提取工艺的优化及其抗氧化活性研究,可为白及多糖提取及综合利用提供借鉴。Objective:To optimize the ultrasonic extraction process of Bletillastriata polysaccharides,compare the content of Bletillastriata polysaccharides from different origins,and investigate the stability and antioxidant activity of Bletillastriata polysaccharides.Methods:Polysaccharide yield rate was taken as the investigation index,the material-liquid ratio,ultrasonic temperature,and ultrasonic time was as the investigation factors,L9(34)orthogonal test was designed to optimize the ultrasonic extraction process of Bletillastriata polysaccharides;the content of Bletillastriata polysaccharides was determined by phenol-sulfuric acid method.the differences in polysaccharide content of Bletillastriata polysaccharides in Hanzhong in Shaanxi,Pu′er in Yunnan,Hongjiang in Hunan and Mianyang in Sichuan were investigated.The stability of Bletillastriata polysaccharides was investigated by chemical methods,and the clearance rate of 1,1-diphenyl-2-picridyl radical(DPPH·)and hydroxyl radical(·OH)by Bletillastriata polysaccharides was compared to evaluate its in vitro antioxidant activity.Results:The optimum extraction conditions were:solid/liquid ratio 1∶25(g/mL),ultrasonic temperature 80℃,ultrasonic time 10 min.The content of Bletillastriata polysaccharides in Mianyang in Sichuan was the highest,reaching 60.81%,Hongjiang in Hunan Province was the second,Puer in Yunnan Province was the lowest;the stability test showed that the stability of Bletillastriata polysaccharides in citric acid and neutral solution was better than that in sodium benzoate,acid or alkaline solution.Bletillastriata polysaccharides can effectively remove DPPH and hydroxyl free radicals,and have potential antioxidant activity in vitro.Conclusion:Study on the optimization of the extraction process of Bletillastriata polysaccharides and its antioxidant activity can provide a reference for the extraction and comprehensive utilization of Bletillastriata polysaccharides.

关 键 词：白及 多糖 超声波 稳定性 抗氧化活性 提取工艺 正交试验 自由基 

分 类 号：R282[医药卫生—中药学]