不同比例构树与苜蓿混合对安格斯母牛瘤胃细菌多样性的影响  

Effects of different combination ratios of paper mulberry and alfalfa on rumen bacterial diversity in Angus cows

作　　者：陈丽娟[1] 李道捷 张云华[2] CHEN Lijuan;LI Daojie;ZHANG Yunhua(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,Anhui,China;School of Resources and Environment,Anhui Agricultural University,Hefei 230036,Anhui,China)

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽农业大学资源与环境学院,安徽合肥230036

出　　处：《草业科学》2020年第8期1579-1587,共9页Pratacultural Science

基　　金：国家自然基金“土壤微生物多样性对豆–禾牧草混播的响应及其与牧草产量和品质的关系(31872418)”;安徽省重点研究与开发项目“芽孢杆菌RBS ALDH基因功能鉴定及其参与棉酚降解机理(201904b11020043)”。

摘　　要：选取两头带瘘管的20~24月龄安格斯母牛作为瘤胃液供体,利用16s rRNA测序技术,采用体外发酵的方法研究不同比例构树(Broussonetia papyrifera)与苜蓿(Medicago sativa)混合发酵对瘤胃细菌多样性的影响。按质量比将试验分为0∶1(DA)、1∶3(D3A1P)、1∶1(D1A1P)、3∶1(D1A3P)、1∶0(DPM)5个处理组。发酵96 h后,收集瘤胃液,进行细菌多样性测序分析。α-多样性指数分析结果显示,各组间Shannon和Simpon指数无显著差异(P>0.05);D1A3P、D3A1P、D1A1P组Chao1指数显著高于DA和DPM组(P<0.05);D1A3P组ACE指数显著高于其他组(P<0.05)。群落结构分析结果显示,在门水平上优势菌为厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、变形杆菌(Proteobacteria)、广古菌门(Euryarchaeota)、螺旋体菌门(Spirochaetes)。DA、D1A1P和DPM组中厚壁菌门相对丰度显著高于其他两组(P<0.05);D1A3P组变形杆菌相对丰度显著高于其他处理组(P<0.05);D3A1P组广古菌门相对丰度显著高于其他处理组(P<0.05)。在属水平上,D1A1P组未注释的拟杆菌属(unidentified_Bacteroidales)的相对丰度显著高于其他处理组(P<0.05);DPM组苏特拉菌属(Sutterella)和解琥珀酸菌属(Succiniclasticum)相对丰度显著高于其他处理组(P<0.05)。不同比例苜蓿和构树在门和属水平上改变瘤胃细菌相对丰度。Rumen fluid was collected from two ruminally fistulated Angus cows aged 20 to 24 months.Using 16S rRNA sequencing technology,the effects of mixed in vitro fermentation of alfalfa and paper mulberry at different ratios on rumen bacterial diversity were studied.According to the mass ratio,the experimental materials were divided into five treatment groups:0∶1(DA),1∶3(D3A1P),1∶1(D1A1P),3∶1(D1A3P),and 1∶0(DPM).Rumen fluid was collected for detection,and bacterial diversity was analyzed after fermentation for 96 hours.Shannon and Simponα-diversity index was not significantly different across the five groups(P>0.05).Chao1 indices of D1A3P,D3A1P,and D1A1P were significantly higher than those of DA and DPM(P<0.05).ACE index of D1A3P was significantly higher than that of the other groups(P<0.05).Community structure analysis showed that the dominant phyla were Firmicutes,Bacteroidetes,Proteobacteria,Euryarchaeota,and Spirochaetes.The relative abundance of Firmicutes in DA,D1A1P,and DPM was significantly higher than that in the other two groups(P<0.05).The relative abundance of Proteobacteria in D1A3P was significantly higher than that in the other groups(P<0.05).The relative abundance of Euryarchaeota in D3A1P was significantly higher than that in the other groups(P<0.05).At the genus level,the relative abundance of unidentified Bacteroidales in D1A1P was significantly higher than that in the other groups(P<0.05).The relative abundance of Sutterella and Succiniclasticum in DPM was significantly higher than that in the other groups(P<0.05).At the phylum and genus levels,the relative abundance of bacterial in the rumen changed with differing ratios of alfalfa and paper mulberry.

关 键 词：构树 苜蓿 体外发酵 瘤胃细菌多样性 

分 类 号：S823.5[农业科学—畜牧学]