编码基因pac的DNA疫苗经鼻粘膜免疫定菌鼠的研究  

Intranasal immunization against dental caries with plasmid DNA encoding pac gene of Streptococcus mutans in gnotobiotic rats

作　　者：郭继华[1] 樊明文[1] 边专[1] 贾荣[1] 彭彬[1] 

机构地区：[1]武汉大学口腔医学院牙体牙髓科,430079

出　　处：《中华口腔医学杂志》2002年第6期452-455,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目 ( 39770 799)

摘　　要：目的　检测pCIA PDNA防龋疫苗经鼻粘膜免疫定菌鼠的效果 ,并对两种不同的载体系统进行对比。方法　将 30只出生后 2 0d断乳的SD雌鼠随机分为 6组 ,制备定菌模型。分别用裸DNA(A组 )、DNA Dosper复合体 (B组 )、DNA Bupivacaine复合体 (C组 )、pCI质粒 (D组 )及无菌水 (E组 )经鼻粘膜免疫大鼠 ,裸DNA股四头肌注射 (F组 )为阳性对照。 2周后加强免疫 1次。 70d鼠龄时收集唾液、血清及粪便 ,酶联免疫吸附实验检测特异性抗体 ,处死大鼠进行Keyes记分 ,单因素方差分析法分析结果。结果　B、C、F组血清特异性抗PAcIgG水平和B、C组唾液中特异性抗PAcIgA抗体水平明显高于其他组 (P <0 0 1 )。疫苗免疫组龋齿记分明显低于阴性对照组 (P <0 0 1 ) ,其中B、C组效果最好。结论　编码基因pac的pCIA PDNA防龋疫苗经鼻粘膜途径进行免疫可以有效预防龋病的发生 。Objectives To assess the efficacy of plasmid DNA encoding pac gene of Streptococcus mutans (S. mutans) intranasally immunized in gnotobiotic rats and to compare the effect of two different delivery systems. Methods Sprague Dawley rats, infected with S. mutans at 20 days of age, were intranasally immunized with plasmid pCIA P (group A), Dosper DNA complex (group B), Bupivacaine DNA complex (group C). Control rats were either immunized with plasmid pCI (group D), distilled water (group E) or immunized intramuscularly (group F). All the rats were boosted 2 weeks later. ELISA determined the antibodies against the vaccines. Keyes caries score was used to evaluate the anti caries effectiveness of the vaccines at the terminal study. Results As for the antibody reactions, there were significantly ( P < 0 01) differences between rats immunized with DNA vaccine and non immunized rats. And rats in group B and C had the significantly ( P < 0 01) higher level of specific salivary anti PAc IgA antibodies and rats(group B?C?F) had the significantly ( P < 0 01) higher specific serum anti PAc IgG responses to DNA vaccine. Keyes scores of rats (group B and C) were significantly ( P < 0 01) lower than others. Conclusions Intranasal immunization with plasmid pCIA P encoding pac gene successfully reduces the caries and appears to be a promising approach against dental caries. Cationic liposome Dosper and local anesthetic bupivacaine could enhance the efficacy of DNA vaccine.

关 键 词：编码基因pac DNA疫苗 龋齿 变异链球菌 疾病预防 粘膜免疫 

分 类 号：R780.1[医药卫生—口腔医学]