miRNA-381靶向PTEN对胃癌细胞增殖与凋亡的影响及相关机制研究  

Effects of miRNA-381 targeting PTEN on proliferation and apoptosis of gastric cancer cells and related mechanisms

作　　者：崔琴[1] 屈振杰[2] 陈吉[1] CUI Qin;QU Zhen-jie;CHEN Ji(Department of Gastroenterology,Baogang Hospital,Baotou Inner Mongolia 014010,China;Department of Oncology,Baogang Hospital,Baotou Inner Mongolia 014010,China)

机构地区：[1]内蒙古包钢医院消化内科,内蒙古包头014010 [2]内蒙古包钢医院肿瘤内科,内蒙古包头014010

出　　处：《临床和实验医学杂志》2020年第18期1930-1933,共4页Journal of Clinical and Experimental Medicine

基　　金：内蒙古自治区自然科学基金(编号:2017MS0879)。

摘　　要：目的探讨与研究miRNA-381靶向10号染色体上磷酸酶与张力蛋白同源缺失基因(PTEN)对胃癌细胞增殖与凋亡的影响及相关机制。方法将处于对数生长期的BGC-823细胞平均分为三组:对照组、实验1组与实验2组,分别转染miRNA NC 20 nmol/L与miRNA-381 mimic 20 nmol/L、40 nmol/L。采用MTT法检测细胞增殖情况,流式细胞仪检测细胞凋亡情况,Transwell小室实验检测细胞迁移情况,实时定量PCR检测miRNA-381表达情况,Western blotting检测PTEN蛋白表达情况。结果转染后24 h、48 h,实验1组与实验2组的细胞增殖指数、细胞迁移指数、miRNA-381表达量高于对照组,差异均具有统计学意义(P<0.05);实验2组高于实验1组,两组比较差异具有统计学意义(P<0.05)。实验2组与实验1组的细胞凋亡指数、PTEN蛋白相对表达量均显著低于对照组,实验2组也较实验1组显著降低,差异均具有统计学意义(P<0.05)。与转染后24 h相比,转染后48 h三组细胞增殖指数、凋亡指数、迁移指数以及miRNA-381相对表达量均显著增加,而PTEN蛋白相对表达量仅对照组显著增加,两个实验组均显著降低(P<0.05)。结论在胃癌细胞中,miRNA-381可通过靶向抑制PTEN表达,从而促进胃癌细胞增殖与迁移,抑制胃癌细胞的凋亡。Objective To explore and study the effects and related mechanism of miRNA-381 targeting phosphatase and tension protein homologous deletion gene deleted from chromosome 10(PTEN)on the proliferation and apoptosis of gastric cancer cells.Methods BGC-823 cells in logarithmic growth phase were divided into three groups-control group:control group,experiment 1 group and experiment 2 group.The control group,experiment 1 group and experiment 2 group were transfected with miRNA NC 20 nmol/L and miRNA-381 mimic 20 nmol/L,40 nmol/L.MTT method was used to detect cell proliferation,flow cytometry to detect cell apoptosis,Transwell cell test to detect cell migration,real-time quantitative PCR to detect miRNA-381 expression,and Western blotting to detect PTEN protein expression.Results At 24 h and 48 h after transfection,cell proliferation index,cell migration index and the miRNA-381 expression of the experimental group 1 and the experimental group 2 were higher than those of the control group(P<0.05),and the experimental group 2 were higher than the experimental group 1,the differences were statistically significant(P<0.05).The relative expression levels of apoptosis index and PTEN protein in the experimental group 2 and the experimental group 1 were lower than those in the control group(P<0.05),and the experimental group 2 were also lower than the experimental group 1,compared the differences were statistically significant(P<0.05).Compared with 24 h after transfection,the cell proliferation index,apoptosis index,migration index and relative expression of miRNA-381 in the three groups at 48 h after transfection were significantly increased.However,the relative expression of PTEN protein increased significantly only in the control group,and decreased significantly in both experimental groups(P<0.05).Conclusion miRNA-381 can inhibit the expression of PTEN in gastric cancer cells,thereby promote the proliferation and migration of gastric cancer cells and inhibit the apoptosis of gastric cancer cells.

关 键 词：胃癌 miRNA-381 10号染色体上磷酸酶与张力蛋白同源缺失基因 细胞增殖 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]