IL-1β基因多态性与汉族人群抑郁障碍相关性研究  

A correlational study between the polymorphism of interleukin-1βgene and depression disorder in Han nationality

作　　者：高雅[1,2] 李申 李洁[1,3] GAO Ya;LI Shen;LI Jie(Clinical College of Mental Health,Tianjin Medical University,Tianjin 300222,China;Department of Psychology,Tianjin Third Central Hospital,Tianjin 300170,China;Laboratory of Biological Psychiatry,Institute of Mental Health,Tianjin Mental Health Center,Tianjin Anding Hospital,Tianjin 300222,China)

机构地区：[1]天津医科大学精神卫生临床学院,天津300222 [2]天津市第三中心医院心理科,天津300170 [3]天津市安定医院,天津市精神卫生中心,天津市精神卫生研究所生物精神病学研究室,天津300222

出　　处：《天津医科大学学报》2020年第5期434-439,共6页Journal of Tianjin Medical University

基　　金：天津市自然科学基金资助项目(16JCYBJC24200);天津市慢性病防治科学重大专项(17ZXMFSY00070)。

摘　　要：目的:探讨白细胞介素(IL)-1β基因多态性与汉族人群生活事件及抑郁障碍的关系。方法:共纳入重性抑郁障碍患者433例作为病例组,并纳入基本情况相匹配的正常对照者421名作为对照组。以HAMD-17方法分别评定两组受试者抑郁障碍的严重程度,同时以生活事件量表(LES)对病例组近1年的生活事件进行评定。从被试者静脉血中提取基因组DNA,选取IL-1β基因上3个位点:rs13032029、rs1143623和rs3917368。采用以基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)进行候选基因多态性位点基因分型。运用SPSS22.0统计软件包进行数据分析。结果:在病例组和对照组中,IL-1β基因rs13032029位点的基因型频率分别为:CC型28.6%和28.5%,CT型47.3%和52.7%,TT型23.8%和18.5%,差异无统计学意义(χ~2=4.028,P=0.258)。在病例组和对照组中,rs1143623位点基因型频率分别为:CC型36.5%和31.1%,GC型45.3%和51.8%,GG型18.0%和16.6%,差异无统计学意义(χ^2=4.29,P=0.232)。在病例组和对照组中,rs3917368位点基因型频率分别为:CC型24.5%和25.4%,CT型47.6%和46.6%,TT型27.3%和27.6%,差异无统计学意义(χ^2=0.302,P=0.960)。3个位点不同基因型患者的HAMD总分、各条目之间的差异没有统计学意义。LES总分及生活事件的多元回归分析差异无统计学意义。结论:未发现IL-1β基因rs13032029、rs1143623和rs3917368基因多态性与汉族人群生活事件和抑郁障碍的发病存在相关性。Objective: To investigate the relationship between interleukin(IL)-1 beta gene polymorphism and life events and depressive disorder in Han population. Methods: A total of 433 patients with major depressive disorder were included as the case group, and421 normal controls matched with the basic conditions were included as the control group. The severity of depressive disorder was evaluated by HAMD-17 method, and the life events of the case group in the past year were evaluated by Life Event Scale(LES).Genomic DNA was extracted from the venous blood of the subjects and three loci on the IL-1β gene were selected: rs13032029, rs1143623 and rs3917368. Genotyping of candidate gene polymorphic loci was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS). SPSS22.0 Statistical package was applied for data analysis. Results: In case group and control group,the genotype frequencies of rs13032029 locus of IL-1β gene were 28.6% and 28.5% for CC type, 47.3% and 52.7% for CT type, 23.8% and18.5% for TT type, respectively. The difference was not statistically significant( χ~2=4.028, P=0.258). In case group and control group, the genotype frequencies of rs1143623 locus were 36.5% and 31.1% for CC type, 45.3% and 51.8% for GC type, 18.0% and 16.6% for GG type,respectively. The difference was not statistically significant(χ^2=4.29, P=0.232). In case group and control group, the genotype frequencies of rs3917368 locus were 24.5% and 25.4% for CC type, 47.6% and 46.6% for CT type, 27.3% and 27.6% for TT type, respectively. The difference was not statistically significant(χ^2=0.302, P=0.960). There was no significant difference in HAMD total score and items among patients with different genotypes at three loci. There was no significant difference in LES total score and life events by multiple regression analysis. Conclusion: The polymorphisms of the IL-1β rs13032029, rs1143623 and rs3917368 are not found to be associated with the occurrence of life events and depressive disorder

关 键 词：重性抑郁障碍 白细胞介素-1Β 基因多态性 生活事件 

分 类 号：R749[医药卫生—神经病学与精神病学]