促甲状腺激素受体与TTF-1、Pax-8在甲状腺乳头状癌中的表达及意义  

Expression and significance of TSHR,TTF-1 and Pax-8 in papillary thyroid carcinoma

作　　者：柳林[1,2] 王丽哲 张永庆[3] 李如江[4] 庄宝祥[5] Liu Lin;Wang Lizhe;Zhang Yongqing;Li Rujiang;Zhuang Baoxiang(Department of Endocrinology, Weifang People′s Hospital, Weifang 261041, China;Department of Internal Medicine, Weifang Medical University, Weifang 261053, China;Department of Thyroid Surgery, Weifang People′s Hospital, Weifang 261041, China;Department of Histoembryology, Weifang Medical University, Weifang 261053, China;Department of Morphology, Weifang Medical University, Weifang 261053, China)

机构地区：[1]潍坊市人民医院内分泌科,山东潍坊261041 [2]潍坊医学院内科学教研室,山东潍坊261053 [3]潍坊市人民医院甲状腺外科,山东潍坊261041 [4]潍坊医学院组织与胚胎学教研室,山东潍坊261053 [5]潍坊医学院形态学教研室,山东潍坊261053

出　　处：《中国医院统计》2020年第4期322-325,共4页Chinese Journal of Hospital Statistics

基　　金：潍坊市科技发展计划项目(2019YX004);山东省医药卫生发展项目(2015WS0076)。

摘　　要：目的探讨促甲状腺激素受体(TSHR)、甲状腺特异性转录因子-1(TTF-1)、成框基因8编码的转录因子(Pax-8)在甲状腺乳头状癌(PTC)、桥本氏病(HT)合并甲状腺乳头状癌、结节性甲状腺肿(NG)与正常甲状腺组织中的表达及意义。方法收集2018年9月至2019年6月在某院内分泌科确诊并在甲状腺外科行手术治疗的甲状腺组织,包括单纯PTC 16例、HT合并PTC 24例,NG 32例及癌旁正常甲状腺组织40例。将术中获得的甲状腺组织进行脱水、透明、包埋处理,分别行TSHR、TTF-1、Pax-8的免疫组织化学染色,显微镜下观察染色结果。采用χ^2检验进行多组间率的比较,差别有统计学意义时采用χ^2分割做两两比较。结果1)TSHR、TTF-1在甲状腺组织的强阳性表达在4组间的差别均有统计学意义(P<0.001,P=0.004),Pax-8在甲状腺组织的强阳性表达在4组间的差别无统计学意义(P=0.642)。2)调整检验水准α′=0.008,进一步两两比较发现:TSHR在PTC组的强阳性表达低于在NG组与癌旁正常组织(P<0.001,P=0.002),在HT合并PTC组的强阳性表达低于在NG组(P=0.001),TTF-1在HT合并PTC组的强阳性表达低于在NG组(P=0.002);其他组两两比较均无统计学意义(P>0.008)。3)在全部PTC组织中,TSHR、TTF-1的阳性表达与性别、年龄、肿瘤直径、淋巴结转移等均无关;Pax-8的阳性表达在有淋巴结转移者低于无淋巴结转移者(P=0.030),与性别、年龄、肿瘤直径无关。结论TSHR与TTF-1在PTC中的强阳性表达低于NG和癌旁正常甲状腺组织,Pax-8在不同类型甲状腺组织中表达无差别。Pax-8在PTC组织中的低表达易出现淋巴结转移。Objective To investigate the expression and significance of thyrotropic-stimulating hormore receptor(TSHR),thyroid transcription factor-1(TTF-1),paired box gene-8(Pax-8)in papillary thyroid carcinoma(PTC),Hashimoto′s disease(HT)with PTC,nodular goiter(NG)and normal thyroid tissues.Methods From September 2018 to June 2019,thyroid tissues confirmed in the Endocrine Department of our hospital and operated in the Thyroid Surgery were collected,including 16 cases of simple PTC,24 cases of HT combined with PTC,32 cases of nodular goiter and 40 cases of normal thyroid tissues adjacent to the carcinoma.The thyroid tissues obtained during the operation were treated with dehydration,transparency and embedding.Immunohistochemical staining of TSHR,TTF-1 and Pax-8 was performed,and the staining results were observed under the microscope.The Chi-square test was used to compare the intergroup rates,and when the difference was statistically significant,Chi-square partition was used to make pairwise comparison.Results 1)There were significant difference about the strong positive expression of TSHR and TTF-1 among four groups of thyroid tissue(P<0.001,P=0.004),while no significant difference in the strong expression of Pax-8 were found among four groups of thyroid tissue(P=0.642).2)After adjusting the statistical examination levelα′=0.008,pairwise comparison showed that the strong positive expression of TSHR in PTC group was lower than that in NG and normal thyroid tissue(P<0.001,P=0.002);the strong positive expression of TSHR in HT combined with PTC group was lower than that in NG(P=0.001);while the strong positive expression of TTF-1 in HT combined with PTC group was lower than that in NG(P=0.002).However,there was no significant difference of the strong positive expression of TSHR,TTF-1 in other groups respectively(P>0.008).3)The positive expression of TSHR,TTF-1 were not related to age,tumor diameter and lymph node metastasis.However,the positive expression of Pax-8 inpatients with lymph node metastasis was lower than tha

关 键 词：甲状腺乳头状癌 自身免疫性甲状腺疾病 促甲状腺激素受体 甲状腺特异性转录因子-1 成框基因8编码的转录因子 

分 类 号：R195.4[医药卫生—卫生统计学]