钼作用下油菜对镉胁迫的生理生化响应及其对镉吸收的特征  

Physiological and biochemical responses to cadmium stress for rape(Brassica napus L.)under molybdenum application and corresponding cadmium absorption characteristics

作　　者：韩张雄 和文祥[1] 王曦婕 熊英[2] HAN Zhangxiong;HE Wenxiang;WANG Xijie;XIONG Ying(Key Laboratory of Plant Nutrition and Agro-environment in Northwest China,Ministry of Agriculture,College of Natural Resources and Environment,Northwest A&F University,Yangling 712100;Xi′an Testing and Quality Supervision Center for Geological and Mineral Products,Ministry of Land and Resources,Shaanxi Institute of Geology and Mineral Resources Experiment Co.,Ltd.,Xi′an 710054)

机构地区：[1]西北农林科技大学资源环境学院,农业部西北植物营养与农业环境重点实验室,杨凌712100 [2]陕西省地质矿产实验研究所有限公司,国土资源部西安矿产资源监督检测中心,西安710054

出　　处：《环境科学学报》2020年第9期3463-3472,共10页Acta Scientiae Circumstantiae

基　　金：国家重点研发计划项目(No.2017YFF0206804);陕西省自然科学基础研究项目(No.2014JM5217)。

摘　　要：为了探讨施钼对不同浓度镉胁迫油菜的缓解效果,利用盆栽试验,研究了4种外源钼梯度(0、50、100、200 mg·kg-1)对低镉(0.5 mg·kg-1)和高镉(6.0 mg·kg-1)两种胁迫下油菜生理生化、生物量、镉的转运富集等的影响作用.结果显示,外源钼可促进镉胁迫油菜长度、鲜重、干重增加.不同浓度的钼可使镉胁迫油菜生理生化指标丙二醛(MDA)、脯氨酸(Pro)积累量显著降低,可溶性蛋白(SP)含量、可溶性糖(SS)含量、叶绿素(Chla和Chlb)含量、超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GPX)活性显著提高;同时,施入钼能够有效抑制油菜对镉的吸收富集,但对其转运影响相对较小,同时又可显著提高油菜对镉的耐性.钼对镉的抑制作用表现为提高油菜的生物量水平、改善其生理生化性能,通过参与油菜生理过程降低油菜对镉的吸收,进而提高油菜对进入体内镉的耐受力,外源钼能有效地降低Cd胁迫对油菜的毒害.To explore the mitigation effect of molybdenum application on cadmium-stressed rape,the effects of 4 exogenous molybdenum gradients(0,50,100 and 200 mg·kg-1)on biomass,physiological biochemistry,cadmium transport and enrichment for rape under low cadmium(0.5 mg·kg-1)and high cadmium(6.0 mg·kg-1)were studied using pot experiment.The results showed that exogenous molybdenum could increase the length,wet weight and dry weight of rape under cadmium stress.Molybdenum application with different concentration could decrease the accumulation of malondialdehyde(MDA)and proline(Pro)in cadmium-stressed rape significantly(p<0.05).While the indexes including soluble protein(SP),soluble sugar(SS),chlorophyll(Chla and Chlb)content,superoxide dismutase(SOD)activity and glutathione peroxidase(GPX)activity increased significantly with molybdenum application(p<0.05).In addition,molybdenum application could effectively inhibit the cadmium absorption and enrichment for rape,but it just slightlyaffected its transport processes.However,the cadmium tolerance of rape was significantly improved,characterized by biomass level of rape,improving its physiological and biochemical performances,reducing the cadmium absorption through participating in the physiological processes of rape,and thus improving its tolerance to cadmium.In conclusion,exogenous molybdenum can effectively reduce the toxic effect of cadmium stress on rape.

关 键 词：外源钼 生理参数 生物量 镉吸收 

分 类 号：X171.5[环境科学与工程—环境科学]