秦川牛ANGPTL3基因g.-38T>C和g.509A>G的遗传多样性及其与生长和胴体性状的关联性分析  

Genetic Diversity of g.-38T>C and g.509A>G in ANGPTL3 and Their Association Analysis with Growth and Carcass Traits in Qinchuan Cattle(Bos taurus)

作　　者：李亚星 成功[2] 赵云博 王申元[3] 吴凯峰[3] 成立新[4] 刘剑锋[5] 周欢敏[3] 李光鹏 昝林森[2] 佟彬 LI Ya-Xing;CHENG Gong;ZHAO Yun-Bo;WANG Shen-Yuan;WU Kai-Feng;CHENG Li-Xin;LIU Jian-Feng;ZHOU Huan-Min;LI Guang-Peng;ZAN Lin-Sen;TONG Bin(State Key Laboratory of Reproductive Regulation&Breeding of Grassland Livestock/College of Life Sciences,Inner Mongolia University,Hohhot 010071,China;National Beef Cattle Improvement Center/College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China;Inner Mongolia Key Laboratory of Biomanufacture/College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010031,China;Animal Husbandry Institute,Inner Mongolia Academy of Agricultural&Animal Husbandry,Hohhot 010031,China;Key Laboratory of Animal Genetics,Breeding,and Reproduction/National Engineering Laboratory for Animal Breeding,Ministry of Agriculture/College of Animal Science and Technology,China Agricultural University,Beijing 100193,China)

机构地区：[1]内蒙古大学省部共建草原家畜生殖调控与繁育国家重点实验室/生命科学学院,呼和浩特010071 [2]西北农林科技大学国家肉牛改良中心/动物科技学院,杨凌712100 [3]内蒙古农业大学内蒙古自治区生物制造重点实验室/生命科学学院,呼和浩特010011 [4]内蒙古农牧业科学院畜牧研究所,呼和浩特010031 [5]中国农业大学农业部畜禽遗传育种与繁殖重点实验室/农业部畜禽育种国家工程实验室/动物科学技术学院,北京100193

出　　处：《农业生物技术学报》2020年第9期1543-1553,共11页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2018YFD0501700);内蒙古自治区应用技术研究与开发资金计划(20180260);人社部高层次留学人才回国资助项目(人社厅函[2018]190号);内蒙古自治区“草原英才”工程(CYYC6068);内蒙古大学高层次人才引进科研启动金(21400-5165112)。

摘　　要：肉牛(Bos taurus)的生长速度和胴体性状在肉牛产业中具有重要的经济价值。血管生成素样蛋白3(angiopoietin-like protein 3,ANGPTL3)在动物脂质、葡萄糖和能量代谢反应中具有关键作用。已有研究表明,ANGPTL3基因上存在的g.-38T>C和g.509A>G突变位点与肉牛生长和胴体性状显著相关。为探究ANGPTL3基因g.-38T>C和g.509A>G位点对秦川牛(Qinchuan cattle)(B.taurus)生长和胴体性状的影响及其在不同黄牛群体中的多样性,本研究利用直接测序和MassARRAY技术,对599头牛—陕西秦川牛384头、山东鲁西黄牛(Luxi cattle)24头、内蒙古蒙古牛(Mongolia cattle)41头、蒙古国蒙古牛(Mongolia cattle)50头、湖南巫陵牛(Wuling cattle)50头和广西隆林牛(Longlin cattle)50头的ANGPTL3基因g.-38T>C和g.509A>G位点进行基因分型和遗传多样性分析,并对其与秦川牛生长和胴体性状的关联性进行分析。结果表明,ANGPTL3基因g.-38T>C和g.509A>G位点在6个黄牛群体中均符合Hardy-Weinberg平衡;在秦川牛群体中,g.-38T>C位点存在TT(0.695)、TC(0.261)和CC(0.044)3种基因型,该位点与眼肌面积和肌内脂肪极显著相关(P<0.01),与体斜长、腰角宽、胸围和背膘厚显著相关(P<0.05);该位点在秦川牛群体中的C等位基因频率与其他5个黄牛群体存在不同程度的显著差异。在秦川牛群体中,g.509A>G位点存在AA(0.690)、AG(0.279)和GG(0.031)3种基因型,该位点与胸围和眼肌面积极显著相关(P<0.01),与体斜长、腰角宽、坐骨端宽和肌内脂肪显著相关(P<0.05);该位点在秦川牛群体中的A等位基因频率与其他5个黄牛群体存在不同程度的显著差异。g.-38T>C和g.509A>G位点联合分析结果显示,TC和AG基因型个体的眼肌面积和肌内脂肪显著高于TT和AA基因型个体(P<0.05)。综上所述,ANGPTL3基因g.-38T>C和g.509A>G位点与秦川牛的生长和胴体性状显著相关。本研究可为秦川牛分子标记辅助育种提供有效分子遗传标记,为秦The growth rate and carcass quality of beef cattle(Bos taurus)have important economic value in beef cattle industry.Angiopoietin-like protein 3 gene(ANGPTL3)is known to be a kind of secreted protein,and plays an important role in lipid,glucose and energy metabolisms.A previous study reported that there were g.-38T>C and g.509A>G mutations identified in ANGPTL3 gene,which were associated with growth and carcass traits in beef cattle.The aims of this study were to investigate the genetic diversity of g.-38T>C and g.509A>G mutations in different cattle populations,and confirm their association with growth and carcass traits in Qinchuan cattle(B.taurus).Total of 599 cattle sample(including 384 Qinchuan cattle,24 Luxi cattle,41 Mongolia cattle from Inner Mongolia,50 Mongolia cattle from Mongolia,50 Wuling cattle and 50 Longlin cattle)were collected for this study.All of 599 samples were genotyped by direct sequencing and MassARRAY technologies,and then the analyses of genetic diversity in 6 cattle populations and association with growth and carcass traits in Qinchuan cattle were performed.The results showed that the genotype frequency of g.-38T>C and g.509A>G in ANGPTL3 were consistent with Hardy-Weinberg equilibrium(P<0.05)in each of 6 cattle populations.There were 3 genotypes TT(0.695),TC(0.261)and CC(0.044)of g.-38T>C mutation in Qinchuan cattle population.Association analysis results showed that g.-38T>C SNP was significantly associated with body length,hip width,chest circumference and back fat thickness(P<0.05),as well as ultrasound loin muscle area and intramuscular fat content(P<0.01)in Qinchuan cattle.Furthermore,there were 3 genotypes AA(0.690),AG(0.279)and GG(0.031)of g.509A>G mutation in Qinchuan cattle population.Association analysis results showed that g.509A>G SNP was significantly associated with body length,pin bone width,hip width and intramuscular fat content(P<0.05),as well as chest circumference and ultrasound loin muscle area(P<0.01).The combination analysis of two SNPs showed that the individual

关 键 词：秦川牛 血管生成素样蛋白3基因(ANGPTL3) 生长性状 胴体性状 分子标记 

分 类 号：S8[农业科学—畜牧兽医]