油菜杂交种宁杂21号及其双亲苗期在盐胁迫条件下差异表达基因分析  

Genes differentially expression in rapeseed hybrid Ningza 21 and its parents at seedling stage under salt tolerance

作　　者：郭月 于艳芳[2,3] 龙卫华 杜建厂[3] 刘静[3] 付丽[1] 浦惠明[2,3] 张洁夫[2,3] 高建芹[2,3] 袁娜[3] 胡琼[1] 胡茂龙[2,3,4] GUO Yue;YU Yan-fang;LONG Wei-hua;DU Jian-chang;LIU Jing;FU Li;PU Hui-ming;ZHANG Jie-fu;GAO Jian-qin;YUAN Na;HU Qiong;HU Mao-long(Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Nanjing Sub-center,National Center of Oil Crops Improvement/Key Laboratory of Cotton and Rapeseed(Nanjing),Ministry of Agriculture and Rural Affairs,Nanjing 210014,China;Provincial Key Laboratory of Agrobiology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Institute of Life Sciences,Jiangsu University,Zhenjiang 212023,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]江苏省农业科学院经济作物研究所/农业农村部长江下游棉花与油菜重点实验室/国家油料作物改良中心南京分中心/江苏省现代作物生产协同创新中心,江苏南京210014 [3]江苏省农业科学院种质资源与生物技术研究所/江苏省农业生物学重点实验室,江苏南京210014 [4]江苏大学生命科学研究院,江苏镇江212023

出　　处：《中国油料作物学报》2020年第4期603-612,共10页Chinese Journal of Oil Crop Sciences

基　　金：中国农科院油料所农业农村部油料作物生物学与遗传育种重点实验室开放课题(KF2018005);国家重点研发计划(2016YFD0101300);国家农业现代产业技术体系建设专项(CARS-12);江苏省农业生物学重点实验室开放课题项目(4911707Z201810)。

摘　　要：为了解甘蓝型油菜杂种优势在盐胁迫中的表现,遏制减产,以油菜强优势杂交种宁杂21号及其亲本为材料,通过转录组测序,筛选鉴定得到1078个芽苗期差异表达基因(DEG)与耐盐杂种优势密切相关,并能被已有盐胁迫相关QTL注释,部分基因的表达能被荧光定量PCR验证,说明所得DEG具有可靠性,有潜在的油菜耐盐育种价值。杂交组合中耐盐杂种优势相关DEG表达模式与母本更为类似,大部分表现为正调控,功能倾向于生物合成的同化作用,而表达模式类似父本的DEG其功能倾向于氧化还原等调节作用。本研究还对耐盐性杂种优势特异的SNP位点进行了预测和注释,以期为杂交油菜耐盐分子标记的开发以及耐盐育种提供理论依据。To understand rapeseed(Brassica napus L.)heterosis on salt tolerance, hybrid cultivar Ningza 21 was used to investigate genes differentially expression compared with its parental lines. Through the next-generation high-throughput transcriptome sequencing technology with no salt treatments as negative control, 1078 differentially expressed genes(DEGs) were obtained, which were recognized to be closely related to heterosis of salt tolerance in oilseed rape at early seedling stage. These DEGs were annotated by released salt stress related QTLs, and expression of some DEGs were verified by qPCR, indicating the reliability of these DEGs. Additionally, the hybrid had more up-regulation candidate DEGs in shoot than those in root, and the expression pattern of DEGs was much closer to that of the maternal line than the paternal line in hybrid Ningza 21. More DEGs in hybrid similar to female parent showed positive regulation, and their function tended to be assimilation of biosynthesis. In contrast, DEGs similar to male parent were much related to redox and other regulatory functions. It was predicted that SNP loci were specific for heterosis of salt tolerance. All the results were expected to be helpful for salt tolerance improvement of hybrid rapeseed.

关 键 词：甘蓝型油菜 耐盐 杂种优势 转录组 遗传机制 

分 类 号：S565.4[农业科学—作物学]