多囊蛋白在成釉细胞瘤的表达及分布研究  

Expression and distribution of polycystin in ameloblastoma

作　　者：侯亚丽 杨丽 刘冰 张昊 李向军 HOU Ya-li;YANG Li;LIU Bing;ZHANG Hao;LI Xiang-jun(Department of Oral Pathology, School and Hospital of Stomatology, Hebei Medical University, Hebei Key Laboratory of Stomatology, Shijiazhuang,050017,China;Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology, Hebei Medical University, Hebei Key Laboratory of Stomatology, Shijiazhuang, 050017, China;Department of Oral Dentistry, School and Hospital of Stomatology, Hebei Medical University, Hebei Key Laboratory of Stomatology, Shijiazhuang 050017, China)

机构地区：[1]河北医科大学口腔医学院,口腔医院病理科,河北省口腔医学重点实验室,河北石家庄050017 [2]河北医科大学口腔医学院,口腔医院口腔颌面外科,河北省口腔医学重点实验室,河北石家庄050017 [3]河北医科大学口腔医学院,口腔医院牙周一科,河北省口腔医学重点实验室,河北石家庄050017

出　　处：《河北医科大学学报》2020年第9期1079-1083,F0003,共6页Journal of Hebei Medical University

基　　金：河北省科技厅重点研发计划项目(17277745D)。

摘　　要：目的观察多囊蛋白1(polycystin-1,PC-1)与多囊蛋白2(polycystin-2,PC-2)在成釉细胞瘤的表达及分布,并与牙源性角化囊肿相比较,为研究成釉细胞瘤的发生和发展机制,寻找新的治疗方法提供线索。方法用免疫组织化学方法检测PC-1和PC-2在30例成釉细胞瘤和15例牙源性角化囊肿中的表达与分布。结果与牙源性角化囊肿组比较,成釉细胞瘤组PC-1和PC-2阳性表达率明显增高(P<0.05),主要分布于成釉细胞瘤中成釉细胞样细胞、部分星网状层样细胞及发生鳞状化生和囊性变的肿瘤细胞的细胞浆、细胞膜及牙源性角化囊肿上皮衬里的棘层、粒层和部分基底细胞。实性/多囊型与单囊型及多囊型成釉细胞瘤的各病理亚型中PC-1和PC-2分布及表达差异均无统计学意义(P<0.05)。结论多囊蛋白可能参与成釉细胞瘤的上皮-间质转化,其高表达可能与成釉细胞瘤的发生发展、肿瘤的囊性转化及生物学行为有关,而与囊肿的形成无明显相关。Objective To observe the expression and distribution of polycystin-1(PC-1)and polycystin-2(PC-2)in ameloblastom,and compared with the odontogenic keratocysts,so as to provide clues for the study of the pathogenesis and development of ameloblastoma and the search for new treatment methods.Methods The expression and distribution of PC-1 and PC-2 in 30 ameloblastomas and 15 odontogenic keratocysts were detected by immunohistochemistry.Results Compared with odontogenic keratocyst group,the positive expression rate of PC-1 and PC-2 in ameloblastoma group was significantly higher(P<0.05).The two proteins were distributed in the cytoplasm and membrane of ameloblast like cells,some stellate reticular like cells,squamous metaplasia and cystic tumor cells in ameloblastoma,as well as in the echinoderm,granular layer and some basal cells lined with odontogenic keratocyst epithelium.There was no difference in the distribution and expression of PC-1 and PC-2 between solid or multicystic and monocystic ameloblastoma and multicystic ameloblastoma(P<0.05).Conclusion Polycystin may be involved in the epithelial stromal transformation of ameloblastoma,and its high expression may be related to the occurrence and development of ameloblastoma,the cystic transformation and biological behavior of the tumor,but not to the formation of cyst.

关 键 词：成釉细胞瘤 牙源性囊肿 多囊蛋白 

分 类 号：R739.82[医药卫生—肿瘤]