柚皮素对高糖模型MIN6细胞的增殖和胰岛素分泌的影响  

Effect of naringenin on cell proliferation and insulin secretion in high hlucose model of MIN6 cells

作　　者：王元 曾凯宏[1,2] 邓波 余雪梅[1] 周雪[1] 宋怡 黄璐娇[1] WANG Yuan;ZENG Kaihong;DENG Bo;YU Xuemei;ZHOU Xue;SONG Yi;HUANG Lujiao(Department of Clinical Nutrition,Sichuan Academy of Medical Sciences & Sichuan Provincial People′s Hospital,Chengdu,Sichuan 610072,China;School of Medicine,University of Electronic Science and Technology of China,Chengdu,Sichuan 610054,China)

机构地区：[1]四川省医学科学院·四川省人民医院临床营养科,成都610072 [2]电子科技大学医学院,成都610054

出　　处：《重庆医学》2020年第17期2844-2848,共5页Chongqing medicine

基　　金：国家自然科学基金项目(81202206);四川省卫生和计划生育委员会项目(150216)。

摘　　要：目的观察不同浓度柚皮素对高浓度葡萄糖(简称“高糖”)模型MIN6细胞增殖活性和胰岛素分泌的影响,以进一步探讨柚皮素对高糖环境下胰岛β细胞胰岛素分泌的分子机制。方法用高糖培养基培养MIN6细胞建立高糖损伤模型,分别用不同浓度(0、25、50、100μg/mL)的柚皮素对模型细胞进行干预,用噻唑蓝(MTT)检测细胞的增殖活性,ELISA检测胰岛素释放能力。结果3个浓度(25、50、100μg/mL)的柚皮素干预均可恢复高糖模型MIN6细胞的增殖活力,可抑制高糖模型MIN6细胞胰岛素分泌能力下降,并且柚皮素浓度为50μg/mL时效果最明显(P<0.01)。结论柚皮素可能作为保护胰岛β细胞功能的潜在降糖药物被临床使用。Objective To observe the effects of different concentrations of naringenin on the proliferative activity and insulin secretion of high glucose model MIN6 cells,and to lay the foundation for further exploring of the effect of naringenin on the insulin secretion of pancreatic islet cells in a high glucose environment.Methods MIN6 cells were cultured in high glucose medium to establish the high glucose damage model.The model cells were treated with different concentrations(0,25,50,100μg/mL)of naringenin,and the proliferation activity of cells was detected by MTT.The ability of insulin secretion was measured by ELISA.Results The intervention of 3 concentrations of naringenin restored the proliferative activity of MIN6 cells which damaged by high glucose,inhibited the decrease of insulin secretion ability of high glucose model MIN6 cells,and the effect was most obvious when the naringenin concentration was 50μg/mL(P<0.01).Conclusion Naringenin may be used as a potential hypoglycemic agent to protect isletβ-cell function.

关 键 词：柚皮素 细胞增殖 胰岛素分泌 MIN6细胞 高糖模型 

分 类 号：R151.2[医药卫生—营养与食品卫生学]