罗非鱼致病性普通变形杆菌的分离鉴定  

Isolation and identification of pathogenic Proteus vulgaris from tilapia

作　　者：黄杰 安佳星 易弋[2] 罗福广 文衍红 胡大胜[3] 韩书煜 HUANG Jie;AN Jiaxing;YI Yi;LUO Fuguang;WEN Yanhong;HU Dasheng;HAN Shuyu(Liuzhou Fishery Technical Extension Station,Liuzhou 545006,China;College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;Guangxi Fishery Technical Extension Station,Nanning 530022,China)

机构地区：[1]柳州市渔业技术推广站,广西柳州545006 [2]广西科技大学生物与化学工程学院,广西柳州545006 [3]广西壮族自治区水产技术推广总站,广西南宁530022

出　　处：《畜牧与兽医》2020年第9期94-98,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31560728);广西自然科学基金(2018GXNSFAA050116)。

摘　　要：从患病罗非鱼体内分离得到1株具有溶血性的革兰阴性杆菌,经16S rDNA系统进化树分析和生理生化鉴定,确认该菌株为普通变形杆菌(Proteus vulgaris)。人工感染试验显示,该菌可导致罗非鱼患病死亡,对于体重50 g左右的罗非鱼,半致死量在1.3×10~6~1.3×10~7 CFU/尾之间。该菌对环丙沙星、依诺沙星、恩诺沙星、头孢曲松等11种药物高度敏感,对多黏菌素B、新生霉素、强力霉素等9种药物完全耐药。恩诺沙星作为水产养殖允许使用的抗菌药物,可用于治疗普通变形杆菌引起的罗非鱼细菌性疾病。A strain of hemolytic gram-negative bacterium was isolated from diseased tilapia.The strain was determined as Proteus vulgaris by 16 S rDNA phylogenetic tree analysis and biochemical identification.The results of artificial infection showed that the strain might cause the death of tilapia.For tilapia with a body weight of of about 50 g,the median lethal dose was between 1.3×10~6 CFU/tail and 1.3×10~7CFU/tail.This bacterium was highly sensitive to 11 drugs,including ciprofloxacin,ilofloxacin,enrofloxacin and ceftriaxone,and so on,and was of complete resistance to 9 drugs,such as polymyxin B,neomycin,doxycycline,and so on.Enrofloxacin,as a permitted antibiotic in aquaculture,might be used to treat the bacterial diseases of tilapia caused by Proteus vulgaris.

关 键 词：罗非鱼 普通变形杆菌 分离与鉴定 致病性 药敏试验 

分 类 号：S96[农业科学—水产养殖]