酚磺乙胺对酶法检测血清尿酸的影响  

Effect of etamsylate on enzymatic method for detecting serum uric acid

作　　者：井沆[1] 牛志杰 刘子砚[1] 凌晓午[1] 李青青 武红权[1] JING Hang;NIU Zhijie;LIU Ziyan;LING Xiaowu;LI Qingqing;WU Hongquan(Department of Clinical Laboratory,Guizhou Provincial People′s Hospital,Guiyang,Guizhou 550002,China;Department of Oncology,Shougang Shuigang General Hospital,Liupanshui,Guizhou 553000,China)

机构地区：[1]贵州省人民医院检验科,贵州贵阳550002 [2]首钢水钢总医院肿瘤科,贵州六盘水553000

出　　处：《检验医学与临床》2020年第18期2641-2644,共4页Laboratory Medicine and Clinic

基　　金：贵州省人民医院青年基金项目(GZSYQN201708)。

摘　　要：目的探讨酚磺乙胺对酶法检测血清尿酸(UA)干扰的临床研究。方法参照美国临床和实验室标准化协会(CLSI)制定的EP7-A2文件,对酶法检测血清UA进行干扰筛选试验和剂量效应试验,通过酚磺乙胺对不同水平UA检测的干扰效应来确定酚磺乙胺对酶法检测UA的影响。结果干扰筛选试验:干扰配对试验组均值为207.33μmol/L,对照样品均值为496.86μmol/L,试验样品的干扰值95%CI为(-226.53±1.96)μmol/L,当干扰值95%CI最低值的绝对值大于预设的干扰试验的可接受标准时,说明酚磺乙胺对酶法检测UA存在严重的干扰。剂量效应试验:低值UA标本,干扰效应回归曲线方程为Y=-2.474e-6 X^3+0.0024 X^2-0.777 X-3.614(F=1596.39,R^2=0.995,P<0.001),当酚磺乙胺水平在29.84 mg/L以上时,干扰值的绝对值高于干扰试验的可接受标准;而高值UA样品,干扰剂量效应回归方程Y=-3.175e-6 X^3+0.00333 X^2-1.294 X-9.832(F=1582.615,R^2=0.996,P<0.001),当酚磺乙胺水平在44.43 mg/L以上,干扰值的绝对值高于干扰试验的可接受标准。结论酚磺乙胺对酶法检测血清UA存在负干扰,在低、高水平UA样品中,酚磺乙胺的水平分别在<29.84 mg/L和<44.43 mg/L时,负干扰程度未超过干扰试验的可接受标准。在临床工作中须采取措施以避免这种干扰。Objective To conduct the clinical study on the interference of etamsylate on the enzymatic method for detecting serum uric acid(UA).Methods Referring to the EP7-A2 document formulated by the American Society for Clinical and Laboratory Standardization(CLSI),the interference screening experiments and dose-response experiments were performed on the enzymatic method for detecting serum UA,and the effect of etamsylate on the enzymatic method for detecting serum UA was determined by the interference effect of etamsylate on different concentrations of UA detection.Results The interference screening experiment:the average value of the interference pairing test group was 207.33μmol/L,the average value of the control sample was 496.86μmol/L,the interference value 95%CI of the test sample was(-226.53±1.96)μmol/L.When the interference value when the minimum lower limit value of interference value 95%CI was greater than the presupposed acceptable standard of the interference experiment,indicating that etamsylate had a serious interference to the enzymatic method for detecting UA.The dose effect experiment:low-value UA samples,the regression curve equation of interference effect Y=-2.474e-6 X^3+0.0024 X^2-0.777 X-3.614(F=1596.39,R^2=0.995,P<0.001),when the etamsylate level was above 29.84 mg/L,the interference value was higher than the acceptable standard of the interference experiment;for the high-value UA samples,the interference dose effect regression equation Y=-3.175e-6 X^3+0.00333 X^2-1.294 X-9.832(F=1582.615,R^2=0.996,P<0.001).When the etamsylate level was above 44.43 mg/L,the interference value was higher than the acceptable standard of the interference experiment.Conclusion Etamsylate has the negative interference on the enzymatic method for detecting serum UA.In the high and low concentrations of UA samples,the etamsylate level was<29.84 mg/L and<44.43 mg/L respectively,the negative interference degree does not exceed the acceptable standard of the interference experiment.The measures should be taken to avoid su

关 键 词：酚磺乙胺 酶法 血清尿酸 药物干扰 

分 类 号：R446[医药卫生—诊断学]