宫颈癌组织中Ⅲ型和Ⅴ型胶原蛋白的表达水平研究  

Study on the expression of typeⅢandⅤcollagen in cervical cancer tissue

作　　者：艾尔肯·肉孜比拉力 焦谊[1] 木叶斯尔·买买提 林晨 Aierken Rouzibilali;JIAO Yi;Muyesier Maimiati;LIN Chen(Department of Biochemistry and Molecular Biology,School of Pre-clinical Medicine,Xinjiang Medical University,Urumqi 830011,China;Department of Pathology,School of Pre-clinical Medicine,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学基础医学院生物化学暨分子生物学教研室,乌鲁木齐830011 [2]新疆医科大学基础医学院病理学教研室,乌鲁木齐830011

出　　处：《新疆医科大学学报》2020年第9期1176-1179,1191,共5页Journal of Xinjiang Medical University

基　　金：国家自然科学基金地区项目(81660428)。

摘　　要：目的探讨宫颈癌发生与Ⅲ型、Ⅴ型胶原蛋的关系。方法收集宫颈癌新鲜组织标本36例和对照组织25例,提取总RNA后,利用实时荧光定量PCR方法对COL3A1、COL5A1和COL5A2 mRNA进行定量分析。另选取宫颈癌和对照组组织石蜡包埋切片各30例,通过免疫组化方法检测COL3A1蛋白表达水平。结果与对照组相比,宫颈癌组织中COL3A1 mRNA表达水平下调,差异具有统计学意义(P<0.05),但COL5A1和COL5A2 mRNA表达水平差异无统计学意义(P>0.05)。COL3A1蛋白在宫颈癌组织中表达下调,与对照组相比差异有统计学意义(P<0.05)。结论宫颈癌组织中COL3A1基因和蛋白的表达水平下调。Objective To investigate the relationship between cervical cancer and typeⅢ,Ⅴcollagens.Methods Thirty-six fresh cervical cancer tissue samples and twenty-five normal control tissue samples were collected,after the extraction of total RNA,COL3A1,COL5A1 and COL5A2 mRNA were analyzed by real-time PCR.In addition,30 cases of cervical cancer and 30 cases of normal control group Paraffin embedded tissues were selected,and the expression level of COL3A1 was detected by immunohistochemistry.Results Compared with the control group,there was a significant down-regulation in the expression of COL3A1 mRNA in the cervical cancer(P<0.05),but there was no significant difference in the expression of COL5A1 and COL5A2 mRNA(P>0.05).The expression of COL3A1 protein in the cervical cancer was significantly lower than in the control group(P<0.05).Conclusion The gene and protein expressions of COL3A1 are down-regulated in the cervical cancer.

关 键 词：宫颈癌 胶原蛋白 COL3A1 COL5A1 COL5A2 

分 类 号：R737.33[医药卫生—肿瘤]