光谱法研究替诺昔康与小牛胸腺DNA相互作用  

Interaction between tenoxicam and calf thymus DNA by spectroscopy

作　　者：马喆[1] MA Zhe(Department of Pharmacy,Shengjing Hospital China Medical University,Shenyang 110004,China)

机构地区：[1]中国医科大学附属盛京医院药学部,辽宁沈阳110004

出　　处：《现代药物与临床》2020年第8期1532-1536,共5页Drugs & Clinic

摘　　要：目的研究替诺昔康与小牛胸腺DNA(ctDNA)之间的相互作用。方法借助紫外光谱和荧光光谱考察了替诺昔康与ctDNA之间的相互作用,同时通过热变性研究、黏度法试验考察了替诺昔康与ctDNA的相互作用模式。结果ctDNA加入使替诺昔康紫外光谱出现轻微减色效应,且随着ctDNA浓度的增大,减色效应变强;替诺昔康的加入使ctDNA-盐酸小檗碱发生荧光猝灭,且其猝灭程度具有浓度相关性。替诺昔康对于ctDNA溶液的黏度和热变性温度影响不大。结论替诺昔康与ctDNA是以沟槽方式结合,并且替诺昔康可以使ctDNA-盐酸小檗碱发生静态荧光猝灭。Objective To study the interaction between tenoxicam(TNX)and calf thymus DNA(ctDNA).Methods The interaction mechanism and mode of TNX with ctDNA were investigated by UV-vis absorption and fluorescence spectroscopy,and DNA thermal denaturation and viscosimetric methods were also adopted.Results After adding ctDNA,the UV-vis spectroscopy of TNX showed a slight hypochromicity,which was further intensified with the increase of ctDNA concentration.As the same time,TNX could quench the fluorescence of ctDNA-berberine hydrochloride and its effect was concentration dependent.However,the relative viscosity and melting of ctDNA didn’t show significant change with the adding of TNX solution.Conclusion The binding between TNX and ctDNA is groove mode,and TNX quenches the fluorescence of ctDNA-BR by static quenching.

关 键 词：替诺昔康 小牛胸腺DNA 相互作用 沟槽结合 

分 类 号：R969.2[医药卫生—药理学]