Simultaneous Quantification of κ-Carrageenan Oligosaccharides of DP 3, 5 and 7 by LC-MS/MS: Application to an in vitro Absorption Study  

作　　者：YANG Yali ZHANG Pengpeng LIU Guilin YANG Shuang WANG Yuanhong JIANG Tingfu LV Zhihua YU Mingming 

机构地区：[1]School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266003,China [3]Key Laboratory of Glycoscience&Glycotechnology of Shandong Province,Qingdao 266003,China [4]Key Laboratory of Marine Drugs,Ministry of Education of China,Qingdao 266003,China

出　　处：《Journal of Ocean University of China》2020年第5期1177-1182,共6页中国海洋大学学报（英文版）

基　　金：supported by the Shandong Provincial Natural Science Foundation,China (No. ZR2019BC025);Open Research Fund Program of Shandong Provincial Key Laboratory of Glycoscience&Glycotechnology (Ocean University of China);the Fundamental Research Funds for the Central Universities (Nos. 201912008,201964019)。

摘　　要：Carrageenans are widely utilized in many commercial applications such as the food and pharmaceutical industry, due to their excellent functional properties. In this study, a sensitive LC-MS/MS method was developed to determine κ-3, κ-5, and κ-7 carrageenan oligosaccharides simultaneously. Optimum MRM transitions for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were(645.079→565.111, [M-H]-),(515.137→474.946, [M-2H]^2-), and(471.484→445, [M-3H]^3-), respectively. Chromatographic separation was performed on an Amide column coupled with a guard column operated at 60℃ under stepwise gradient elution. The linearity of the LC-MS/MS method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides, evaluated over the concentration range of 0.10- 20.0 μmol L^-1, was excellent. The precisions of the method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were from 0.91% to 9.66%, and the inter-day precisions were from 0.92% to 10.5%. Validation of the LC-MS/MS method indicated that the method was precise and in line with the CFDA guidance. This method has been successfully applied to an in vitro absorption study.

关 键 词：κ-carrageenan oligosaccharide Caco-2 cell LC-MS/MS degree of polymerization 

分 类 号：R914[医药卫生—药物化学]