过表达Mfn2基因通过PI3K/AKT通路抑制鱼藤酮诱导的帕金森病细胞模型的细胞凋亡  

The overexpression of Mfn2 inhibits rotenone induced cell apoptosis of Parkinson’s disease cell model through PI3K/Akt pathway

作　　者：张琼 叶丽丽 Zhang Qiong;Ye Lili(Department of Neurology,Anhui Provincial Armed Police Corps Hospital,Hefei 230041;不详)

机构地区：[1]武警安徽省总队医院神经内科,合肥230041 [2]皖南医学院第二附属医院神经内科

出　　处：《卒中与神经疾病》2020年第4期435-439,443,共6页Stroke and Nervous Diseases

摘　　要：目的探讨过表达线粒体融合蛋白2(Mfn2)基因通过PI3K/AKT通路抑制鱼藤酮诱导的帕金森病细胞模型的细胞凋亡。方法培养SH-SY5Y细胞并分组,对照组用不含药物及质粒的DMEM干预;鱼藤酮组用含有20μmol/L鱼藤酮的DMEM干预;鱼藤酮+空白质粒组用含有20μmol/L鱼藤酮的DMEM干预并转染空白的pcDNA 3.1质粒;鱼藤酮+Mfn2质粒组用含有20μmol/L鱼藤酮的DMEM干预并转染表达Mfn2的pcDNA3.1质粒;鱼藤酮+Mfn2质粒+LY组用含有20μmol/L鱼藤酮、10μmol/L LY294002的DMEM干预并转染表达Mfn2的pcDNA3.1质粒;检测细胞活力、凋亡率及线粒体凋亡基因、p-PI3K、p-AKT的表达水平。结果鱼藤酮组的细胞活力及细胞中p-PI3K、p-AKT的表达水平均低于对照组,凋亡率及细胞中caspase-9、cleaved caspase-3的表达水平均高于对照组;鱼藤酮+Mfn2质粒组的细胞活力及细胞中p-PI3K、p-AKT的表达水平均高于鱼藤酮组,凋亡率及细胞中caspase-9、cleaved caspase-3的表达水平均低于鱼藤酮组;鱼藤酮+Mfn2质粒+LY组的细胞活力及细胞中p-PI3K、p-AKT的表达水平均低于鱼藤酮+Mfn2质粒组,凋亡率及细胞中caspase-9、cleaved caspase-3的表达水平均高于鱼藤酮+Mfn2质粒组。结论过表达Mfn2基因对鱼藤酮诱导的帕金森病细胞模型的细胞凋亡具有抑制作用,且该作用可能与抑制PI3K/AKT通路有关。Objective To study the inhibition effect of overexpression of Mfn2 on rotenone induced apoptosis of Parkinson’s disease cell model through PI3 K/Akt pathway. Methods SH-SY5 Y cells were cultured and divided into groups. Control group was treated with DMEM without drugs and plasmids, rotenone group was treated with DMEM containing 20 μmol/L rotenone, rotenone+blank plasmids group was treated with DMEM containing 20 μmol/L rotenone and transfected with blank pcDNA 3.1 plasmids, rotenone+Mfn2 plasmids group was treated with DMEM containing 20 μmol/L rotenone and transfected with Mfn2 expressed pcDNA 3.1 plasmids, rotenone+Mfn2 plasmids+LY group was treated with DMEM containing 20 μmol/L rotenone, 10 μmol/L LY294002 and transfected with Mfn2 expressed pcDNA 3.1 plasmids. The cell viability, apoptosis rate and the expression levels of apoptosis gene, p-PIK3 and p-AKT were detected. Results The cell viability and the expression levels of p-PIK3, p-AKT in rotenone group were lower than those in the control group, and the apoptosis rate and the expression levels of caspase-9, cleared caspase-3 were higher than those in the control group. The cell viability and the expression levels of p-PIK3, p-AKT in rotenone+Mfn2 group were higher than those in the rotenone group, and the apoptosis rate and the expression levels of caspase-9, cleared caspase-3 were lower than those in the rotenone group. The cell viability and the expression levels of p-PIK3, p-AKT in rotenone+Mfn2+LY group were lower than those in the rotenone+Mfn2 group, and the apoptosis rate and the expression levels of caspase-9, cleaved caspase-3 were higher than those in the rotenone+Mfn2+group. Conclusion The overexpression of Mfn2 gene had an inhibitory effect on rotenone induced cell apoptosis of Parkinson’s disease cell model, which was related to the inhibition of PI3 K/Akt pathway.

关 键 词：帕金森病 线粒体融合蛋白2 鱼藤酮 凋亡 PI3K/AKT通路 

分 类 号：R742.5[医药卫生—神经病学与精神病学]