长夏环境中松果腺对大鼠小肠差异基因的影响  

Effects of Pineal Gland on Differential Genes in Small Intestines of Rat under the Long Summer Environment

作　　者：刘雷蕾 马淑然[1] LIU Leilei;MA Shuran(Beijing University of Chinese Medicine,Beijing 100029)

机构地区：[1]北京中医药大学,北京市100029

出　　处：《中医杂志》2020年第17期1553-1558,共6页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81774162);北京市自然科学基金(7204276);北京中医药大学基本科研业务费项目(2019-JYB-JS-003)。

摘　　要：目的探讨松果腺在长夏环境中对大鼠肠道基因表达的影响,从转录组学水平初步揭示"脾应长夏"的物质基础。方法 24只SD大鼠随机分为空白组、伪手术组和手术组,每组8只。手术组大鼠结扎矢状静脉、摘除松果腺,伪手术组大鼠结扎矢状静脉但不摘除松果腺,空白组不予处理。术后继续饲养28天。比较各组大鼠一般状态及粪便粒数、含水率,采用转录组测序筛选出手术组大鼠小肠组织中显著差异表达基因,并对差异基因进行GO功能的显著性富集分析以及KEGG通路的显著性富集分析。结果长夏环境中切除大鼠松果腺后,手术组大鼠的饮食及活动度有所减少,体质量明显下降,24h粪便粒数及粪便含水率明显增加(P<0.01)。测序结果显示,手术组大鼠肠道组织中呈现出64个显著差异表达基因,上调基因有20个,下调基因有44个。GO功能富集分析结果提示,这些差异表达基因与T细胞活化、淋巴细胞迁移、白细胞与细胞间黏附以及适应性免疫反应等功能相关。KEGG通路富集分析结果表明这些基因与细胞黏附分子相关途径、自然杀伤细胞介导的细胞毒性、产生IgA的肠道免疫网络、趋化因子信号通路、Th1和Th2细胞分化以及核因子κB(NF-κB)信号通路等相关。结论松果腺可通过调控肠道中Itgb2、Zap70、Cd40、Tnfsf13b和Icam1等免疫相关基因的表达,抑制肠道免疫环境中包括NF-κB信号通络在内的多条免疫途径,促使机体适应长夏环境,从而防止腹泻的发生,提示松果腺是"脾应长夏"的关键所在。Objective To explore the effects of pineal gland on the expression of intestinal gene in rats in long summer, and to reveal the material basis of "spleen corresponding to long summer" from the level of transcriptomics. Methods A total of 24 SD rats were randomly divided into a blank group, a sham operation group and an operation group, with 8 rats in each group. Rats in the operation group were ligated the sagittal vein and removed the pineal gland, rats in the sham operation group were ligated the sagittal vein but did not remove the pineal gland, and the blank group was not treated. The rates were raised for 28 days after operation. The general condition of rats in each group and the number of feces and water content were compared. Transcriptome sequencing was used to screen out the significantly differentially expressed genes in the small intestine tissue of the rats in the operation group, and the significant enrichment analysis of GO function and the KEGG pathway of the differential genes were performed. Significant enrichment analysis. Results After excision of rat pineal gland in long summer environment, the diet and activity of the rats in the operation group were decreased, and their body weight were decreased significantly, while the 24-hour defecation amount and fecal water content increased significantly(P<0.01). Sequencing results showed that there were 64 significant differential genes in the intestinal tissue of the operation group, with 20 up-regulated genes and 44 down-regulated genes. GO functional enrichment analysis indicated that these differentially expressed genes were associated with T cell activation, lymphocyte migration, leukocyte and intercellular adhesion, and adaptive immune response. KEGG pathway enrichment analysis revealed that these genes were associated with cell adhesion molecule-related pathways, natural killer cell-mediated cytotoxicity, IgA-producing intestinal immune network, chemokine signaling pathway, Th1 and Th2 cell differentiation, and NF-κB signaling pathway. Conclu

关 键 词：松果腺 肠道免疫 脾应长夏 核因子ΚB 转录组测序 

分 类 号：R223[医药卫生—中医基础理论]