弱精子症患者精子差异表达蛋白质串联质谱标签技术筛查与生物信息学分析  

Screening and Bioinformatics Analysis of Sperm Differentially Expressed Proteins by Tandem Mass Spectrometry Tags Technology in Patients with Asthenozoospermia

作　　者：张盼盼 努尔比亚·阿力甫 毛吾兰·买买提依明 马文静[3] 西尔艾力·买买提 阿地力江·伊明 夏米西努尔·伊力克[2] 童卓云 ZHANG Pan-pan;Nuerbiya Alifu;Maowulan Maimaitiyiming;MA Wen-jing;Xieraili Maimaiti;Adilijiang Yiming;Xiamixinuer Yilike;TONG Zhuo-yun(Faculty of Human Anatomy,School of Basic Medical Sciences,Xinjiang Medical University,Urumqi 830011,China;Faculty of Biology,Xinjiang Medical University,Urumqi 830011,China;Central Laboratory,Xinjiang Medical University,Urumqi 830011,China;Faculty of Human Patho-physiology,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学基础医学院人体解剖学教研室,乌鲁木齐830011 [2]新疆医科大学基础医学院生物学教研室,乌鲁木齐830011 [3]新疆医科大学基础医学院中心实验室,乌鲁木齐830011 [4]新疆医科大学基础医学院病理生理学教研室,乌鲁木齐830011

出　　处：《国际生殖健康／计划生育杂志》2020年第5期357-360,364,I0001,I0002,共7页Journal of International Reproductive Health/Family Planning

基　　金：国家自然科学基金(81760786);新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2016I029)。

摘　　要：目的:筛查弱精子症患者精子中差异表达蛋白质,进行生物信息学分析,阐明重要的生物学过程、分子功能及相关的代谢通路,为进一步开展机制研究提供方向与思路。方法:分别收集正常人和弱精子症患者各30例的精子样本,利用串联质谱标签技术(Tandem Mass Tag,TMT)筛查和鉴定弱精子症患者精子中的差异蛋白,并对其进行GO和KEGG分析。结果:以P<0.05,且表达倍数≥1.2或≤0.833为标准,与正常人组精子相比表达有差异的蛋白共1020种,上调蛋白606种,下调蛋白414种。GO分析结果显示上述差异表达蛋白主要参与mRNA分解过程、终止翻译过程,在核糖体和部分胞质均有分布,且具有结合功能、受体活性功能等;差异蛋白参与的KEGG信号通路为代谢通路、腺苷酸活化蛋白激酶(AMPK)信号通路和氧化磷酸化通路等257条通路。结论:弱精子症患者差异表达蛋白质涉及到复杂的生物学过程、功能与通路,为进一步开展弱精子症发病机制的研究提供了重要的方向与生物信息。Objective:To screen the differentially-expressed proteins in sperm of patients with asthenozoospermia,to perform bioinformatics analysis,and to elucidate the important biological processes,molecular functions and related metabolic pathways,so as to provide new ideas for further mechanism research.Methods:The sperm samples were collected from 30 normal men(as the control group)and 30 asthenospermia patients,respectively.The differentially-expressed proteins in the asthenospermia group were screened and identified by tandem mass spectrometry tag technology.Bioinformatics analysis was performed by GO and KEGG.Results:With P<0.05 and the expression ratio≥1.2 or≤0.833 as the standard,there were 1020 differently-expressed proteins,including 606 upregulated proteins and 414 downregulated proteins.GO analysis showed that the differentially-expressed proteins were mainly involved in the mRNA decomposition process and the terminating translation process.They were distributed in ribosomes and cytoplasm,with binding function and receptor-activition function.The KEGG signaling pathway analysis showed that the differentially-expressed proteins were involved in 257 pathways including metabolic pathway,adenylate activated protein kinase(AMPK)signaling pathway and oxidative phosphorylation pathway.Conclusions:The Differentially-expressed proteins in patients with asthenozoospermia were involved in complex biological processes,functions and pathways.This study may provide biological information for further research on the pathogenesis of asthenozoospermia.

关 键 词：弱精子症 精子 蛋白质组学 信号传导 计算生物学 

分 类 号：R698.2[医药卫生—泌尿科学]