维生素E促进小鼠H-22移植瘤的作用及机制  

Promoting effect of vitamin E on H-22 transplanted tumor in mice

作　　者：罗雪霞 陈紫航 王思敏 杨艳红[1] LUO Xue-xia;CHEN Zi-hang;WANG Si-min;YANG Yan-hong(The First Affiliated Hospital(School of Clinical Medicine),Guangdong Pharmaceutical University,Guangzhou 510080,China)

机构地区：[1]广东药科大学附属第一医院(临床医学院),广东广州510080

出　　处：《生物技术》2020年第4期368-374,410,共8页Biotechnology

基　　金：国家自然科学基金项目(“复方贞术调脂方通过TLR4/NF-κB通路改善p120-catenin敲低所致肝脏炎症性糖脂代谢紊乱的机制研究”,81803912);广东省教育厅特色创新类项目(自然科学)广东药科大学“创新强校工程”资助项目(“尼古丁通过影响肠道菌群导致糖脂代谢异常及复方贞术调脂方干预机制”,2017KTSCX102);广东省中医药局科研项目(“利用S180荷瘤小鼠模型对黄连素抑制肿瘤分子机制的初步研究”,20182079);广州市越秀区教育卫生专项项目(“复方贞术调脂方改善昼夜节律失调所致糖脂代谢紊乱的作用及机制研究”,2018-WS-011);2019年度广东省大学生创新创业训练计划项目(“基于Hippo/Notch/Fox O信号通路对黄连素抑制胃癌作用的分子机制研究”,S201910573021)。

摘　　要：[目的]利用H-22荷瘤小鼠研究维生素E(vitamin E,VE)对肿瘤发生发展的作用及机制。[方法]建立H-22小鼠荷瘤模型,计算肿瘤指数与肝脏指数,利用试剂盒检测血糖、血清低密度脂蛋白(low density lipoprotein,LDL)、总胆固醇(total cholesterol,TC)以及碱性磷酸酶(alkaline phosphatase,AKP)水平,利用苏木素-伊红染色与Real-time PCR检测VE对肿瘤发展和转移的作用及分子机制。[结果]肿瘤-VE组LDL水平、肿瘤组和肿瘤-VE组TC、AKP水平显著高于对照组(LDL,P <0.05;TC,P <0.01;AKP,P <0.001)。肿瘤组织中肿瘤-VE组小鼠p27K ip1、Stat3以及Cxcl12基因表达水平显著高于肿瘤组(p27Kip1和Stat3,P <0.05,Cxcl12,P <0.01)。肝脏组织中肿瘤-VE组Jak2、Pigf及Cxcl12基因表达水平显著高于肿瘤组(P <0.05),且肿瘤组Jak2、Pigf及Cxcl12基因表达水平显著高于对照组(P <0.05)。[结论] VE处理的H-22荷瘤小鼠肿瘤组织中p27Kip1、Stat3及Cxcl12表达水平显著升高,肝脏组织中Jak2、Pigf及Cxcl12表达水平显著升高,VE可能通过调控细胞周期、肿瘤转移及炎症相关基因的表达促进小鼠H-22肿瘤的发展。[Objective] The effects and underlying mechanisms of vitamin E(VE) in the development and metastasis of tumor were studied in H-22 tumor-bearing mice.[Method] H-22 tumor-bearing mice model was established. Tumor index and liver index were calculated,and blood glucose,serum low density lipoprotein(LDL),total cholesterol(TC) and alkaline phosphatase(AKP) levels were checked using detection kits. Hematoxylin-eosin staining and Real-time PCR were used to study the effects of VE on the development and metastasis of tumor using the H-22 tumor-bearing mice.[Result]The LDL level of the tumor-VE group,the TC and AKP levels of the tumor-VE group and the tumor group were significantly increased compared with the control group(LDL,P < 0. 05;TC,P < 0. 01;AKP,P < 0. 001). The expression of p27 Kip1,Stat3 and Cxcl12 in tumor tissue of the tumor-VE group was significantly upregulated compared with the tumor group(p27 Kip1 and Stat3,P < 0. 05;Cxcl12,P < 0. 01). The expression level of Jak2,Pigf and Cxcl12 in liver tissue of the tumor-VE group was significantly upregulated compared with the tumor group(P < 0. 05),and the expression level of Jak2,Pigf and Cxcl12 in liver tissue of the tumor group was significantly upregulated compared with the control group(P < 0. 05).[Conclusion]The expression of p27 Kip1,Stat3 and Cxcl12 in the tumor tissue of the H-22 bearing mice treated with VE was significantly increased. The expression of Jak2,Pigf and Cxcl12 in the liver tissue of the H-22 bearing mice treated with VE was significantly increased. VE might promote the development and metastasis of tumor by regulating the expression of genes related with cell cycle,inflammation and metastasis.

关 键 词：维生素E H-22荷瘤小鼠 JAK2/STAT3 CXCL12 PIGF P27KIP1 

分 类 号：R730.1[医药卫生—肿瘤]