Chimeric Newcastle Disease Virus-like Particles Containing DC-Binding Peptide-Fused Haemagglutinin Protect Chickens from Virulent Newcastle Disease Virus and H9N2 Avian Influenza Virus Challenge  

作　　者：Xiaohong Xu Jing Qian Lingsong Qin Jindou Li Cong Xue Jiaxin Ding Weiqi Wang Wei Ding Renfu Yin Ningyi Jin Zhuang Ding 

机构地区：[1]Laboratory of Infectious Diseases,College of Veterinary Medicine,Key Laboratory of Zoonosis Research,Ministry of Education,Jilin University,Changchun 130062,China [2]College of Basic Medical Science,Jilin University,Changchun 130021,China [3]Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory for Veterinary Bio-Product Engineering,Ministry of Agriculture,Nanjing 210014,China [4]College of Agriculture and Forestry Science,Linyi University,Linyi 276000,China

出　　处：《Virologica Sinica》2020年第4期455-467,共13页中国病毒学（英文版）

基　　金：supported by grants from the National Key Research and Development Program of China(Grant No.2018YFD0500100);the National Natural Science Foundation of China(Grant Nos.31772735,31472195);the Jiangsu Provincial Natural Science Foundation of China(Grant No.BK20180299);Jiangsu Agriculture Science and Technology Innovation Fund CX(19)3019;the Key Technology Research and Development Project of Jilin Province(Grant No.20180201021NY)。

摘　　要：Newcastle disease virus(NDV)and H9N2 subtype Avian influenza virus(AIV)are two notorious avian respiratory pathogens that cause great losses in the poultry industry.Current inactivated commercial vaccines against NDV and AIV have the disadvantages of inadequate mucosal responses,while an attenuated live vaccine bears the risk of mutation.Dendritic cell(DC)targeting strategies are attractive for their potent mucosal and adaptive immune-stimulating ability against respiratory pathogens.In this study,DC-binding peptide(DCpep)-decorated chimeric virus-like particles(cVLPs),containing NDV haemagglutinin–neuraminidase(HN)and AIV haemagglutinin(HA),were developed as a DC-targeting mucosal vaccine candidate.DCpep-decorated cVLPs activated DCs in vitro,and induced potent immune stimulation in chickens,with enhanced secretory immunoglobulin A(sIgA)secretion and splenic T cell differentiation.40μg cVLPs can provide full protection against the challenge with homologous,heterologous NDV strains,and AIV H9N2.In addition,DCpep-decorated cVLPs could induce a better immune response when administered intranasally than intramuscularly,as indicated by robust s IgA secretion and a reduced virus shedding period.Taken together,this chimericVLPs are a promising vaccine candidate to control NDV and AIV H9N2 and a useful platform bearing multivalent antigens.

关 键 词：Newcastle disease virus-like particles DC-binding peptide H9N2 Secretory immunoglobulin A(sIgA) Candidate vaccine 

分 类 号：S858.31[农业科学—临床兽医学]