曲酸的液相色谱分离方法研究及其在化妆品分析中的应用  

Investigation of liquid chromatography methods on the separation of kojic acid and its application in cosmetics analysis

作　　者：余诗雨 刘育坚 杨怡 吉洋 刘智敏[1] 许志刚[1] 刘宏程[2] YU Shi-yu;LIU Yu-jian;YANG Yi;JI Yang;LIU Zhi-min;XU Zhi-gang;LIU Hong-cheng(Faculty of Science,Kunming University of Science and Technology,Kunming 650500,China;Institute of Quality Standard and Testing Technology,Yunnan Academy of Agriculture Science,Kunming 650223,China)

机构地区：[1]昆明理工大学理学院,云南昆明650500 [2]云南省农业科学院质量标准与检测技术研究所,云南昆明650223

出　　处：《化学研究与应用》2020年第9期1724-1727,共4页Chemical Research and Application

基　　金：国家自然科学基金项目(21565018)资助;云南省重点研发计划项目(2018BC005-04)资助;昆明理工大学大学生课外学术科技创新基金项目(201910674222)资助;昆明理工大学测试基金项目(2019M20172111049、2019B201811104227)资助。

摘　　要：曲酸是面制品和化妆品中常见的增白剂,由于极性强而给分离带来一定的困难。对不同类型的色谱柱进行分离优化,建立了反相高效液相色谱法高效分离曲酸的方法。对色谱柱的串联顺序及色谱分离条件进行了研究,实验结果表明:在C18柱下,流动相为1%醋酸水溶液-甲醇=93∶7(v/v)、柱温25℃、流速0.7 mL·min^-1、检测波长270 nm,曲酸分离效果最佳。该方法能检测出样品中的曲酸,加标回收率在93.89%~119.11%之间。Kojic acid is a common whitening agent in pastas and cosmetics.It is hard to separate from samples due to its high polarity.The chromatographic column was optimized and a reversed phase high performance liquid chromatography method with C18 column and ultraviolet visible detection for kojic acid analysis in cosmetics was established.The chromatographic conditions were as follows:mobile phase was 1%acetic acid water:methanol=93∶7,the flow rate was 0.7ml·min^-1,the column temperature was 25℃and the detection wavelength was 270nm.Kojic acid could be detected in real samples by this method and the recovery was between 93.89%and 119.11%.

关 键 词：C18柱 HILIC柱 曲酸 化妆品分析 

分 类 号：O657.7[理学—分析化学]