青蒿琥酯对乳腺癌细胞的生长抑制作用及其机制  

Inhibitory effect of artesunate on the growth of breast cancer cells and its mechanism

作　　者：杨超[1] 张彦收[1] 张庚 杜凯邺 李静平[1] 刘亮[2] Yang Chao;Zhang Yan-Shou;Zhang Geng;Du Kai-Ye;Li Jing-Ping;Liu Liang(Breast Disease Cancer,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;Tumor Institute,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院乳腺中心,石家庄050011 [2]河北医科大学第四医院肿瘤研究所,石家庄050011

出　　处：《解放军医学杂志》2020年第8期804-809,共6页Medical Journal of Chinese People's Liberation Army

基　　金：河北省自然科学基金(H2017206174);河北省医学科学研究重点课题(20180493,20190696);2017年政府资助临床医学优秀人才培养和基础课题(冀财社[2017]46号);河北省重点研发计划项目(17277766D);河北省普通高等学校强势特色学科肿瘤学建设经费(冀教高[2005]52号)。

摘　　要：目的探讨青蒿琥酯对乳腺癌细胞MDA-MB-231的生长抑制作用及其可能机制。方法利用CCK-8法检测青蒿琥酯对MDA-MB-231细胞的生长抑制作用,并计算IC50值。根据IC50值选择后续实验的青蒿琥酯浓度(0、25、50、100μg/ml),0μg/ml青蒿琥酯组作为对照组。采用不同浓度(0、25、50、100μg/ml)的青蒿琥酯干预MDA-MB-231细胞24 h,利用流式细胞术检测细胞凋亡情况及线粒体膜电位;0、25μg/ml青蒿琥酯干预MDA-MB-231细胞24 h,利用细胞划痕试验检测细胞迁移能力,Transwell实验检测细胞侵袭能力。结果青蒿琥酯对MDA-MB-231细胞具有增殖抑制作用,且呈浓度依赖性,对MDA-MB-231细胞的IC50为54.24μg/ml。不同浓度的青蒿琥酯干预MDA-MB-231细胞24 h,MDA-MB-231细胞凋亡率显著增高(P<0.01);与对照组相比,青蒿琥酯组细胞线粒体膜电位显著降低(P<0.01),且呈浓度依赖性。细胞划痕实验结果显示,青蒿琥酯组细胞迁移能力低于对照组;Transwell实验结果显示,青蒿琥酯可抑制MDA-MB-231细胞的侵袭能力(P<0.01)。结论青蒿琥酯对乳腺癌MDA-MB-231细胞具有生长抑制作用,且抑制其侵袭及迁移能力,其作用机制与调控细胞线粒体膜电位,诱导细胞凋亡作用有关。Objective To investigate the function of artesunate on the growth of breast cancer cell line MDA-MB-231 and its potential mechanism.Methods CCK-8 assays were used to measure growth inhibition in breast cancer cells(MDA-MB-231)in the presence of artesunate,the IC50 was calculated.Based on the IC50 values,various artesunate concentrations(0,25,50,100μg/ml)were used to experiments in this paper.0μg/ml artesunate group served as the control group,using normal saline instead of the artesunate.Apoptosis and mitochondrial membrane potential were analyzed by flow cytometry,after the treatment of various artesunate concentrations(0,25,50,100μg/ml)for 24 hours.Migration and invasion of the MDA-MB-231 cells were evaluated using wound healing and Transwell assays,respectively,after the treatment of various artesunate concentrations(0,25μg/ml)for 24 hours.Results Artesunate inhibited the growth of MDA-MB-231 cells in a dose-dependent manner,with IC50 values for MDA-MB-231 of 54.24μg/ml.Compared to mock-treated cells,artesunate significantly increased apoptosis,while the mitochondrial membrane potential was significantly decreased after 24 h of exposure to different concentrations of artesunate(P<0.01),in a dose-dependent manner.The cell migration and invasion abilities were also lower in the 25μg/ml artesunate treated cells than these abilities in the mock-treated cells.Conclusions Artesunate could inhibit the growth of MDA-MB-231 breast cancer cells and inhibit its invasion and migration ability by inducing apoptosis and decreasing mitochondrial membrane potential.

关 键 词：乳腺癌 细胞凋亡 流式细胞术 线粒体膜电位 青蒿琥酯 

分 类 号：R737.9[医药卫生—肿瘤]