华中五味子藤茎化学成分及其抗氧化活性  

Chemical Constituents of the Canes of Schisandra sphenanthera and Their Antioxidant Activities

作　　者：石新卫[1,2] 路强强 李晓君 周亚福 万一[2] 李为民 SHI Xin-wei;LU Qiang-qiang;LI Xiao-jun;ZHOU Ya-fu;WAN Yi;LI Wei-min(Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources,Xi an Botanical Garden of Shaanxi Province/Institute of Botany of Shaanxi Province,Xi an 710061,Shaanxi,China;Engineering Center of Qinling Mountains Natural Products,Shaanxi Academy of Sciences,Microbiology Institute of Shaanxi,Xi an 710043,Shaanxi,China;School of Chemical Engineering and Technology,North University of China,Taiyuan 030051,Shanxi,China)

机构地区：[1]陕西省植物资源保护与利用工程技术研究中心陕西省西安植物园/陕西省植物研究所,陕西西安710061 [2]陕西省科学院秦岭天然产物工程中心陕西省微生物研究所,陕西西安710061 [3]中北大学化学工程与技术学院,山西太原030051

出　　处：《西北林学院学报》2020年第5期207-212,共6页Journal of Northwest Forestry University

基　　金：陕西省科学院科技计划顶目(2018nk-01);陕西省自然科学基础研究计划项目(2020JM-708);国家自然科学基金(31200257);西安市科技计划项目(20193023YF011NS011,20NYYF0043)。

摘　　要：以华中五味子藤茎为材料,采用硅胶柱层析、MCI树脂柱层析、Sephadex LH-20凝胶柱层析等方法,分离鉴定所得化合物的结构,采用ABTS方法评价其抗氧化活性,以全面了解华中五味子的化学成分,明确其藤茎中药效成分及活性作用。结果表明:1)从藤茎乙酸乙酯萃取部位中分离得到7个化合物,分别鉴定为胡萝卜苷(1)、pinobatol(2)、Matairesinol 4′-O-glucoside(3)、(-)-secoisolariciresinol-O-a-L-rhamnopyranoside(4)、(-)-secoisolariciresinol 9-O-α-L-arabinopyranoside(5)、(-)-儿茶素(6)和异鼠李素-3-O-β-D-葡萄糖苷(7);2)化合物2~5为首次从该属植物中分离得到;3)化合物2~7清除ABTS自由基IC 50值分别为14.43、13.45、7.39、3.84、1.88、14.11μg·mL-1;化合物4~6抗氧化活性与阳性对照品相当。Taking the canes of Schisandra sphenanthera as materials,chemical constituents were thoroughly investigated and their antioxidant activities were evaluated in order to explicit active constituents and action mode.The chemical constituents were isolated and purified by various chromatographic techniques,such as silica column chromatography,MCI resin column chromatographic and Sephadex LH-20 column chromatographic methods.Their structures were elucidated on the basis of spectroscopic methods,and the antioxidant activities of compounds 2-7 were evaluated by ABTS free radical scavenging assay.The results were as follows:1)seven compounds were elucidated on the basis of spectral and chemical evidences,i.e.,daucosterol(1),pinobatol(2),Matairesinol 4′-O-glucoside(3),(-)-secoisolariciresinol-O-α-L-rhamnopyranoside(4),(-)-secoisolariciresinol 9-O-α-L-arabinopyranoside(5),(-)-catechin(6),and isorhamnetin-3-O-β-D-glucoside(7).2)Compounds 2-5 were isolated from Schisandra for the first time.3)Compounds 2-7 presented significant scavenging activities against ABTS free radicals,the IC 50 values of which were 14.43,13.45,2.93,7.39,3.84,1.88μg·mL-1 and 14.11μg·mL-1,respectively.Compounds 4-6 exhibited similar antioxidant activities to positive control.

关 键 词：华中五味子 化学成分 抗氧化活性 ABTS自由基 

分 类 号：R284.1[医药卫生—中药学]