一种新型主客体荧光探针对人体氨基酸的识别研究  

Identification of Human Amino Acids by A Novel Host-Guest Fluorescent Probe

作　　者：蒋道法 杨希楠 金艳梅 高洁[1] 赵威威 马培华[1] Jiang Daofa;Yang Xinan;Jin Yanmei;Gao Jie;Zhao Weiwei;Ma Peihua(Key Laboratory of Macrocyclic Chemistry and Supram olecular Chemistry of Guizhou Province,Guizhou University,Guiyang,550025)

机构地区：[1]贵州大学贵州省大环化学与超分子化学重点实验室,贵阳550025

出　　处：《化学通报》2020年第9期813-820,769,共9页Chemistry

基　　金：国家自然科学基金项目(21762011);贵州省科技计划项目(黔科合平台人才[2017]5788号)资助。

摘　　要：本文研究了环戊基全取代六元瓜环(CyP6Q[6])与2-(β-吡啶)-1H-咪唑[4,5-f][1,10]菲咯啉盐酸盐(即Ar-IPHS)的主客体相互作用,并利用CyP6Q[6]可使Ar-IPHS产生强烈荧光的性质制备了Ar-IPHS@CyP6Q[6]荧光探针用以识别氨基酸。结果表明,探针可高效识别甘氨酸、赖氨酸、甲硫氨酸,检出限分别为2.27×10^-6、1.359×10^-5和1.690×10^-5mol/L。In this paper,the host-guest interaction of cyclopentyl substituted cucurbit[6]uril(CyP6Q[6])and 2-(β-pyridine)-1H-imidazole[4,5-f][1,10]phenanthroline hydrochloride(Ar-IPHS)was studied,and a fluorescent probe Ar-IPHS@CyP6Q[6]was prepared to identify amino acids by utilizing the property that CyP6Q[6]can make Ar-IPHS produce strong fluorescence.The experimental results showed that the probe can efficiently identify glycine,lysine,and methionine with detection limit of 2.27×10^-6 mol/L,1.359×10^-5 mol/L,and 1.690×10^-5 mol/L,respectively.

关 键 词：探针 氨基酸 识别 

分 类 号：R341[医药卫生—基础医学] O657.3[理学—分析化学]