纳米粒子在食源性致病菌检测中的应用进展  

Progress on Analytical Application of Nanoparticles in Foodborne Pathogenic Bacteria

作　　者：邱佩佩 毋福海[1] 白研[1] 贺锦灿 QIU Pei-pei;WU Fu-hai;BAI Yan;HE Jin-can(Guangdong Provincial Engineering Research Center of Public Health Detection and Assessment,Guangdong Pharmaceutical University,Guangzhou 510310,China)

机构地区：[1]广东药科大学广东省公共卫生检测与评价工程中心,广东广州510310

出　　处：《分析测试学报》2020年第9期1160-1167,共8页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21804025);广东省自然科学基金项目(2018A0303130079);广州市科技计划项目(201804010329);广东省教育厅“创新强校工程”项目(2019GCZX012,2017KQNCX105);广东省医学科学技术研究基金项目(A2018004)。

摘　　要：食品安全事关人民群众的身体健康和生命安全,而食源性致病菌是食品安全的主要影响因素。由食源性致病菌引起的疾病和死亡持续威胁着全球的公共卫生安全。因此,开发快速、准确且灵敏的食源性致病菌检测方法是预防食源性疾病暴发和确保食品安全的关键。常规检测方法费时费力,需要昂贵的设备和专业的人员,应用受限。近年来,随着纳米技术的快速发展,纳米粒子凭借其小尺寸、高比表面积和高反应活性等理化特性成为食源性致病菌检测领域的研究热点。此外,将识别元件修饰于纳米粒子表面并结合新颖的分析技术,能提高检测的特异性和灵敏度。该综述主要总结和比较了磁性纳米粒子、贵金属纳米粒子、荧光纳米粒子和二氧化硅纳米粒子在食源性致病菌检测中的应用,以期为食源性致病菌的快速分析提供思路。Food safety is closely related to people's health and life safety,and foodborne pathogenic bacteria is the main factor affecting food safety.Diseases and deaths caused by foodborne pathogenic bacteria continue to threaten global public health.Therefore,development of rapid,accurate and sensitive detection methods for foodborne pathogenic bacteria is the key to prevent foodborne disease outbreaks and ensure food safety.Current conventional detection methods are time-consuming and labor-intensive,which require expensive equipment and professional personnel,and limit their own applications.In recent years,with the rapid development of nanotechnology,nanoparticles have become a research hotspot in the field of foodborne pathogenic bacteria due to their unique physical and chemical properties such as small size,high specific surface area,high reactivity,etc.In addition,the specificity and sensitivity of detection could be improved by modifying the recognition element on the surface of nanoparticles and combining with novel analytical techniques.In this review,the applications of magnetic nanoparticles,noble metal nanoparticles,fluorescent nanoparticles and silica nanoparticles in the detection of foodborne pathogenic bacteria are mainly summarized and compared,aiming at providing a new idea for rapid detection of foodborne pathogenic bacteria.

关 键 词：磁性纳米粒子 贵金属纳米粒子 荧光纳米粒子 二氧化硅纳米粒子 食源性致病菌 检测 

分 类 号：O657.3[理学—分析化学] R155.5[理学—化学]