抑制Livin的表达对喉癌细胞HEP-2增殖的影响  

Effect of Inhibiting the Expression of Livin on the Proliferation of Laryngeal Carcinoma Cell Line HEP-2

作　　者：焦江琴[1] 李捷[1] 朱丽英[1] JIAO Jiangqin;LI Jie;ZHU Liying(The Second Affiliated Hospital of Henan University of Science and Technology,Luoyang,471000)

机构地区：[1]河南科技大学第二附属医院,471000

出　　处：《实用癌症杂志》2020年第9期1464-1467,共4页The Practical Journal of Cancer

基　　金：2018年河南省医学科技攻关计划联合共建项目(编号:2018020921)。

摘　　要：目的探讨抑制Livin的表达对喉癌细胞HEP-2增殖的影响。方法将喉癌细胞系HEP-2分为三组,实验组感染Livin-siRNA重组腺病毒的HEP-2细胞;阴性对照组感染Livin-control重组腺病毒的HEP-2细胞;空白对照组未感染重组腺病毒的HEP-2细胞。采用qRT-PCR检测Livin mRNA表达,MTT检测细胞增殖,流式细胞术检测细胞凋亡,Transwell实验检测细胞迁移,Western blot检测蛋白表达。结果感染48 h与72 h后,与阴性对照组与空白对照组相比,实验组的Livin mRNA表达量和细胞迁移数目显著降低(P<0.05),细胞增殖抑制率和细胞凋亡率显著增高(P<0.05);感染后48 h,与阴性对照组与空白对照组相比,实验组的Livin、Caspase-3蛋白表达显著增高(P<0.05);与感染48 h相比,实验组感染72 h后UHRF1 mRNA相对表达量和细胞迁移数目显著降低(P<0.05),细胞增殖抑制率和细胞凋亡率显著增高(P<0.05),而其余两组两个时间点以上指标则无显著差异(P>0.05)。结论抑制Livin的表达能能激活喉癌细胞的Caspase-3通路,从而抑制喉癌细胞HEP-2增殖与迁移,促进细胞凋亡。Objective To investigate the effect of inhibitions of Livin expression on the proliferation of laryngeal carcinoma cell line HEP-2.Methods The laryngeal carcinoma cell line HEP-2 were divided into 3 groups.The experimental group were infected with Livin-siRNA recombinant adenovirus HEP-2 cells;the negative control group were infected with Livin-control recombinant adenovirus HEP-2 cells;the blank control group were not infected.Livin mRNA expression were detected by qRT-PCR,cell proliferation were detected by MTT,apoptosis were detected by flow cytometry,cell migration were detected by Transwell assay,and protein expression were detected by Western blot.Results After 48 and 72 hours of infection,compared with the negative control group and the blank control group,the expression level of Livin mRNA and the number of cell migration in the experimental group were significantly reduced(P<0.05),and the inhibition rate of cell proliferation and apoptosis rate increased significantly(P<0.05);48 hours after infection,compared with the negative control group and the blank control group,the relative expression of Livin and Caspase-3 protein in the experimental group increased significantly(P<0.05);Compared with the 48-hour infection,the expression of UHRF1 mRNA and the number of cell migration after the 72-hour infection in the experimental group were significantly reduced(P<0.05).and the inhibition rate of cell proliferation and apoptosis rate increased significantly(P<0.05),but there were no significant difference in other 2 groups between the 2 time points(P>0.05).Conclusion Inhibition of Livin expression can activate the Caspase-3 pathway in laryngeal carcinoma cells,thereby inhibiting the proliferation and migration of laryngeal carcinoma HEP-2 and promoting apoptosis.

关 键 词：LIVIN 喉癌 细胞增殖 细胞凋亡 Caspase-3通路 

分 类 号：R739.65[医药卫生—肿瘤]