长链非编码RNA LINC00310在甲基丙烯酸环氧丙酯诱导人支气管上皮细胞恶性转化中的表达改变  

Role of LINC00310 in the glycidyl methacrylate-induced malignant transformation of human bronchial epithelial cell

作　　者：马顺鹏 王全凯[1,2] 谢广云[1] 郭浩然 宋佳阳 乌瀚宝栎尔 许建宁[1,2] Ma Shunpeng;Wang Quankai;Xie Guangyun;Guo Haoran;Song Jiayang;Wuhan Baolier;Xu Jianning(National Institute of Occupational Health and Poison Control,Chinese Center for Disease Control and Prevention,Beijing 100050,China;Key Laboratory of Chemical Safety and Health,Chinese Center for Disease Control and Prevention,Beijing 100050,China;Center for Disease Control and Prevention of Xinbei District,Changzhou,Changzhou 213022,China)

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [2]中国疾病预防控制中心化学污染与健康安全重点实验室,北京100050 [3]常州市新北区疾病预防控制中心,常州213022

出　　处：《卫生研究》2020年第5期711-715,723,共6页Journal of Hygiene Research

基　　金：国家自然科学基金(No.81673221)。

摘　　要：目的探讨LINC00310在甲基丙烯酸环氧丙酯(GMA)诱导人支气管上皮细胞16HBE恶性转化中的表达改变。方法复苏成功的16HBE细胞使用1μg/mL二甲基亚砜作为溶剂对照,终浓度为8μg/mL GMA为染毒组,每次染毒72 h,间隔24 h后再次染毒,重复染毒3次后,分别进行传代培养。取第10、20和30代(前、中和后期)GMA染毒组和溶剂对照组的细胞进行长链非编码RNA芯片分析,观察LINC00310在不同时期的表达改变,同时利用NCBI、cBioPortal生物信息学数据库寻找可能作用靶点,实时荧光定量PCR检测LINC00310和可能作用靶点的相对表达量。结果芯片结果显示,与同时期溶剂对照组相比,第10、20、30代的GMA染毒组LINC00310分别下调2.02倍、上调6.17倍和上调2.03倍。实时荧光定量PCR的验证结果中,三个时期的LINC00310相对表达量变化趋势与芯片分析的结果基本一致,分别下调2.76倍、上调2.68倍和上调3.09倍,可能作用靶点C-Myc相对表达量在第20、30代分别上调3.52倍和下调1.49倍,差异具有统计学意义(P<0.05)。结论LINC00310在GMA诱导16HBE细胞恶性转化的前期低表达,中后期高表达,C-Myc和LINC00310在中期的相对表达量一致,表明LINC00310可通过C-Myc在细胞恶性转化过程中发挥促癌作用。OBJECTIVE To investigate the expression and biological significance of LINC00310 in the malignant transformation of human bronchial epithelial cells(16 HBE)induced by glycidyl methacrylate(GMA).METHODS The 16 HBE cells recovered successfully used 1μg/mL dimethyl sulfoxide(DMSO)as the solvent control group,and the final concentration was 8μg/mL GMA as the treatment group,and were subcultured after repeated exposure 3 times for 72 hours each time.The 10 th,20 th and 30 th generation cells of the GMA treatment group and corresponding DMSO control group were collected.The LncRNA microarrays was used to analyze the expression changes of LINC00310 in different periods,and the target gene and function prediction was performed by NCBI and cBioPortal bioinformatics database,and real-time quantification PCR(qPCR)was used to detect the relative expression levels of LINC00310 and predicted target genes.RESULTS The result of the microarray showed that LINC00310 in the GMA-treated group was down-regulated by 2.02-fold,up-regulated by 6.17-fold,and up-regulated by 2.03-fold in the pre-transformation,mid-term,and late,respectively.The result of qPCR confirmed that the expression of LINC00310 relative expression level of 10 th,20 th and 30 th generation cells was consistent with the microarray result,which were down-regulated by 2.76-fold,up-regulated by 2.68-fold,and up-regulated by 3.09-fold.Consistently,the relative expression of the target gene C-Myc was statistically significant in 20 th and 30 th generation cells.CONCLUSION LINC00310 induced low expression in the early stage of malignant transformation of 16 HBE cells induced by GMA,and was highly expressed in the middle and late stages.It indicated that LINC00310 may play a cancer-promoting role in the process of cell malignant transformation through C-Myc.

关 键 词：长链非编码RNA LINC00310 甲基丙烯酸环氧丙酯 人支气管上皮细胞 细胞恶性转化 

分 类 号：R734.1[医药卫生—肿瘤] R730.23[医药卫生—临床医学]