盐霉素对鼻咽癌CNE-2细胞放射敏感性的影响  

Salinomycin enhances the radioresponse in human nasopharyngeal carcinoma cell lines CNE-2

作　　者：张永芹[1] 左云[1] 宋宇[1] 吕燕 王少开[1] 陈志鹏[1] 卢玮冬[1] 许震[1] Zhang Yongqin;Zuo Yun;Song Yu;Lv Yan;Wang Shaokai;Chen Zhipeng;Lu Weidong;Xu Zhen(Department of Oncology,Zhangjiagang First People's Hospital,Zhangjiagang Hospital Affiliated to Suzhou University,Jiangsu Zhangjiagang 215600,China)

机构地区：[1]张家港市第一人民医院苏州大学附属张家港医院肿瘤科,江苏张家港215600

出　　处：《现代肿瘤医学》2020年第20期3474-3478,共5页Journal of Modern Oncology

基　　金：苏州市科技计划项目(编号:SYSD2016006)。

摘　　要：目的:体外实验研究盐霉素对鼻咽癌CNE-2细胞放射敏感性的影响。方法:通过CCK-8法检测盐霉素作用后鼻咽癌CNE-2细胞的增殖情况。利用克隆集落形成实验观察鼻咽癌细胞成活情况,单击多靶模型拟合剂量存活曲线,计算放射增敏指数(SER);Chou-Talalay数学模型绘制联合指数(CI)曲线,判断盐霉素和放射的作用关系。利用γ-H2AX焦点形成检测DNA分子损伤情况。结果:盐霉素能够抑制鼻咽癌细胞的增殖,并且具有明显的时间和剂量依赖性;盐霉素作用鼻咽癌细胞的IC50值在24 h时为17.81μmol/L,48 h时为3.98μmol/L。根据单击多靶模型拟合细胞的存活曲线,可知在鼻咽癌细胞CNE-2中盐霉素浓度为0.1μmol/L和0.5μmol/L时SER分别为1.19和1.21,均大于1.0,表明盐霉素对鼻咽癌细胞具有明显的放射增敏作用。制作联合指数(CI)曲线,可知在盐霉素浓度为0.1μmol/L时,放射剂量为2 Gy时,CI值≈1,盐霉素和放射对CNE-2的作用接近相加作用,放射剂量为4、6、8 Gy时,CI值均<1,二者为协同作用;在盐霉素浓度为0.5μmol/L时,放射剂量为2、4、6、8 Gy时,CI值均<1,二者均为协同作用。相对于照射组,盐霉素+照射组能增加DNA损伤数目。结论:盐霉素能提高鼻咽癌细胞的放射敏感性,为一种潜在的放射增敏药物。Objective:To evaluate whether salinomycin can change the sensitivity of nasopharyngeal carcinoma cell line CNE-2 to radiotherapy.Methods:The proliferation of CNE-2 cells treated with salinomycin was detected by CCK-8.The cell survival curves were determined by cloning assay and cell clone technique for evaluating the cell lethal effects through multitarget one-lit model.The combination index(CI)curve was drawn by Chou-Talalay method to judge the interaction between salinomycin and radiation.DNA damage was detected byγ-H2 AX foci counting.Results:The salinomycin inhibited the growth of CNE-2 in time-and dose-dependent manner.At 24 h,the IC50 of salinomycin on CNE-2 cells was 17.81μmol/L.At 48 h,the IC50 of salinomycinon on CNE-2 cell lines was 3.98μmol/L.The sensitizing enhancement ratio(SER)was 1.19 for CNE-2 cells which were treated by salinomycin of 0.1μmol/L.After treatment with salinomycin with 0.5μmol/L,SER was 1.21.Both were greater than 1.0,it indicated that salinomycin had obvious radiosensitization effect on nasopharyngeal carcinoma cells.The CI values of salinomycin(0.1μmol/L)was≈1 when combined with radiation(2 Gy),indicated an additivity effect,while the CI values of salinomycin(0.1μmol/L)were both<1 when combined with radiation(4,6,8 Gy)indicated a synergy effect.The CI values of salinomycin(0.5μmol/L)were both<1 when combined with radiation(2,4,6,8 Gy),indicated a synergy effect.Compared with the radiation alone group,salinomycin(0.5μmol/L)combined with radiation(4 Gy)induced more DNA double chain breaks.Conclusion:We provided evidence in vitro that salinomycin enhances radiosensitivity of CNE-2 cells.Salinomycin appears to be a potential radiotherapy sensitization agent for nasopharyngeal carcinoma treatment.

关 键 词：盐霉素 鼻咽癌 放射敏感性 CNE-2细胞 

分 类 号：R739.63[医药卫生—肿瘤]