miR-1271通过靶向FOXF2抑制胃肠道间质瘤细胞的恶性生物学行为  

miR-1271 inhibits the malignant biological behavior of gastrointestinal stromal tumor cells by targeting FOXF2

作　　者：何甜[1] 万苹[1] 郭强[1] 白璇[1] 罗丽琳[2] 杨超[1] 王芸[1] 安瑛[1] HE Tian;WAN Ping;GUO Qiang;BAI Xuan;LUO Lilin;YANG Chao;WANG Yun;AN Ying(Department of Gastroenterology,Yunnan First People′s Hospital/the Affiliated Hospital of Kunming University of Science and Technology,Kunming,Yunnan 650032,China;Department of Pathology,Yunnan First People′s Hospital/the Affiliated Hospital of Kunming University of Science and Technology,Kunming,Yunnan 650032,China)

机构地区：[1]云南省第一人民医院/昆明理工大学附属医院消化内科,昆明650032 [2]云南省第一人民医院/昆明理工大学附属医院病理科,昆明650032

出　　处：《重庆医学》2020年第18期3013-3019,共7页Chongqing medicine

基　　金：云南省应用基础研究项目(2017FE468(-113));云南省科技创新人才计划项目(2018HC006)。

摘　　要：目的探讨微RNA-1271(miR-1271)靶向叉头框转录因子F2(FOXF2)对胃肠道间质瘤(GIST)细胞增殖、侵袭和凋亡的影响。方法采用实时荧光定量PCR(RT-PCR)检测miR-1271及FOXF2在GIST组织和细胞系中的表达水平;Western blot检测FOXF2的表达水平;双荧光素酶报告基因验证miR-1271与FOXF2的靶向关系;采用CCK-8、Transwell、流式细胞术方法分析miR-1271/FOXF2分子轴对GIST-T1细胞增殖、侵袭和凋亡的影响。结果与癌旁组织比较,miR-1271在GIST组织中低表达;与人肠上皮细胞FHs74Int比较,miR-1271在GIST细胞系中也低表达。同时,双荧光素酶报告基因证实,miR-1271可靶向结合FOXF2并下调其表达水平。进一步研究发现,miR-1271通过靶向下调FOXF2可显著抑制GIST-T1细胞增殖、侵袭和诱导凋亡。此外,体内实验同样证实,过表达miR-1271可显著抑制GIST-T1移植瘤瘤体的生长。结论miR-1271通过靶向FOXF2抑制GIST细胞增殖和侵袭能力,同时促进细胞凋亡。Objective To explore the effect of miR-1271 on the proliferation,invasion and apoptosis of gastrointestinal stromal tumors(GIST)cells through targeting FOXF2.Methods The expression of miR-1271 in tissue and cell lines was detected by RT-PCR.The dual luciferase reporter gene assay was used to verify whether FOXF2 was a target gene of miR-1271.Western blot was applied to detect the level of FOXF2.CCK-8,Transwell,flow cytometry methods to analyze the effects of miR-1271/FOXF2 molecular axis on proliferation,invasion and apoptosis of GIST-T1 cells.Results Compared with the adjacent tissues or FHs74Int cells,miR-1271 was lowly expressed in the GIST tissues or cell lines of GIST,the expression level of miR-1271 was significantly downregulated in GIST tissues cell lines of GIST.At the same time,the dual luciferase reporter gene confirmed that miR-1271 could target FOXF2 in GIST-T1 cells and down-regulate its expression level.Furthermore,overexpression of miR-1271 could strongly inhibited proliferation,invasion and induced apoptosis of GIST-T1 cells via downregulating FOXF2.Additionally,in vivo experiments also confirmed that overexpression of miR-1271 could significantly suppressed the growth of GIST xenografts in nude mice.Conclusion miR-1271 suppresses proliferation,invasion and promotes apoptosis of GIST cells via targeting FOXF2.

关 键 词：胃肠肿瘤 miR-1271/FOXF2分子轴 细胞增殖 细胞凋亡 肿瘤侵润 

分 类 号：R735.2[医药卫生—肿瘤]