microRNA调控膜系统及离子转运介导青稞盐碱胁迫的应答  

MicroRNA-mediated Membrane System and Ion Transport are Involved in the Response to Saline-alkali Stress in Tibetan Hulless Barley

作　　者：王玉林[1] 原红军[1] 扎桑[1] 杨春葆 徐齐君[1] WANG Yu-lin;YUAN Hong-jun;ZHA Sang;YANG Chun-bao;XU Qi-jun(State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement&Tibet Academy of Agricultural and Animal Husbandry Sciences/Institute of Agricultural Research,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850002,China)

机构地区：[1]省部共建青稞和牦牛种质资源与遗传改良国家重点实验室/西藏自治区农牧科学院农业研究所,西藏拉萨850032

出　　处：《大麦与谷类科学》2020年第4期7-14,共8页Barley and Cereal Sciences

基　　金：西藏财政专项(2017CZZX002);国家大麦青稞产业技术体系(CARS-05)。

摘　　要：盐碱胁迫是影响作物生长和生产的最严重的非生物胁迫之一。青稞作为青藏高原地区生长的裸大麦,对极端环境适应性强,其盐碱胁迫下microRNA(miRNA)调控机理的研究可为植物盐碱逆境胁迫应答提供重要的参考。本研究利用高通量测序技术,通过对盐碱胁迫及对照处理的青稞进行miRNA测序,挖掘青稞miRNA在盐碱环境下的调控网络。研究鉴定了13757个miRNAs,其中225个在普通大麦中也存在。miRNA靶基因的功能富集分析表明,盐碱胁迫响应miRNAs的潜在靶基因与膜系统及离子转运的生物学过程相关,表明膜系统的形成与离子转运对青稞的盐碱应答发挥着重要的作用。Saline-alkali stress is one of the most serious abiotic stresses that affect crop growth and production.Cultivated in the Tibetan Plateau,Qingke,also called Tibetan hulless barley,adapts well to extreme environmental conditions.Therefore,research on miRNA regulatory network in Qingke under saline-alkali stress can pave the way for elucidation of plant response to abiotic stresses.The present study investigated the miRNA-mediated response to saline-alkali stress in Qingke.By using high-throughput sequencing,13,757 miRNAs were identified,of which 225 were found to be conserved in Hordeum vulgare.The potential target genes of the saline-alkali responsive miRNAs are predicted to be involved in membrane-related and ion transport pathways.These results suggest that membrane formation and ion transport play important roles in Tibetan hulless barley in response to saline-alkali stress.

关 键 词：MIRNA 青稞 盐碱胁迫 

分 类 号：S512.3[农业科学—作物学] Q74[生物学—分子生物学]