BenchMark全自动特殊染色机在肝脏穿刺活检网状纤维染色中的应用  

Application of BenchMark automatic special staining instrument in reticular fiber staining of liver biopsy

作　　者：陈昱[1] 杨眉[1] 王蓉[1] 廖红艳[2] 毛志刚[2] 郑沁[2] CHEN Yu;YANG Mei;WANG Rong;LIAO Hongyan;MAO Zhigang;ZHENG Qin(Department of Pathology,West China Hospital Sichuan University,Chengdu,Sichuan 610041,P.R.China;Department of Laboratory Medicine,West China Hospital Sichuan University,Chengdu,Sichuan 610041,P.R.China)

机构地区：[1]四川大学华西医院病理科,成都610041 [2]四川大学华西医院实验医学科,成都610041

出　　处：《华西医学》2020年第9期1090-1093,共4页West China Medical Journal

基　　金：国家自然科学基金(81902129);四川省科学技术厅项目(2019YFS0321)。

摘　　要：目的探讨BenchMark全自动特殊染色机进行肝脏穿刺(肝穿)组织网状纤维染色的实验条件,评价其染色效果并分享使用经验。方法选取四川大学华西医院2019年4月-5月肝穿标本30例,4%中性缓冲甲醛固定,全自动脱水机处理,石蜡包埋,选用黏附载玻片,切片厚度5μm,65℃烤箱烤片30 min。切片一式两份,一份采用仪器法,按照所在实验室探索的仪器染色条件使用BenchMark全自动特殊染色机进行网状纤维染色;另一份采用手工Foot染色法,按照所在实验室标准化操作规程进行网状纤维染色。染色完成后,请具有丰富阅片经验的主任病理医师评估显微镜下染色效果。结果仪器法30例标本均染色成功,染色成功率为100.0%;手工法30例标本中,23例染色成功,7例染色效果欠佳,染色成功率为76.7%。两种方法染色成功率差异有统计学意义(χ^2=5.143,P=0.023)。结论BenchMark全自动特殊染色机染色效果稳定可靠,成功率优于手工Foot染色法。Objective To explore the dyeing conditions of reticular fiber staining of liver puncture tissue stained by BenchMark automatic special staining instrument,evaluate the staining effect,and share the using experience.Methods A total of 30 cases of liver puncture specimens from April to May 2019 in West China Hospital of Sichuan University were selected.They were fixed with 4%neutral formaldehyde fix solution,treated with automatic dehydrator,embedded in paraffin,stained with adhesive slides,and baked in 65℃oven for 30 min.Each specimen was sliced in duplicate,with a thickness of 5μm.One was reticular fiber stained with BenchMark automatic special staining instrument under the dyeing condition according to the using experience of the laboratory,and the other was reticular fiber stained by manual Foot staining method according to the standard operating procedure of the laboratory.The staining effect under microscope was observed by a chief pathologist with extensive slide-reading experience.Results The success rate of instrumental method was 100.0%(30/30),and that of the manual method was 76.7%(23/30).The difference in success rate between the two methods was statistically significant(χ^2=5.143,P=0.023).Conclusion The reticular fiber staining effect of BenchMark automatic special staining instrument is stable and reliable,and the success rate is higher than that of manual Foot staining.

关 键 词：BenchMark全自动特殊染色机 网状纤维染色 Foot染色法 标准操作规程 

分 类 号：R575[医药卫生—消化系统]