前列腺癌基因表达标志物1在膀胱癌组织的表达变化及其对膀胱癌细胞增殖和凋亡的影响  

Expression of prostate cancer gene expression marker 1 in bladder cancer and its effect on proliferation and apoptosis of bladder cancer cells

作　　者：李征[1] 杨立新[1] 刘磊[1] 朱清[1] 程双蕾 杨小明[2] Li Zheng;Yang Lixin;Liu Lei;Zhu Qing;Cheng Shuanglei;Yang Xiaoming(Department of Urologyy Nanyang Central Hospital,Affiliated Hospital of Zhengzhou University,Nanyang 473000,China;Department of Urology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学附属医院南阳中心医院泌尿外科,473000 [2]郑州大学第一附属医院泌尿外科,450052

出　　处：《中华实验外科杂志》2020年第7期1339-1341,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察前列腺癌基因表达标志物1(PCGEM1)对膀胱癌细胞增殖和凋亡的影响并探讨其分子机制。方法选取2016年6月到2019年6月南阳中心医院收集的159例膀胱癌组织和对应的癌旁组织作为研究对象。采用荧光定量聚合酶链反应(PCR)分析癌旁组织和膀胱癌组织中PCGEM1表达水平。采用RNA干扰技术在膀胱癌细胞T24和BIU-87(购自上海中国科学院细胞库)建立PCGEM1敲降和对照细胞株,分别为PCGEM1 KD/T24组、T24对照组、PCGEM1 KD/BIU-87组和BIU-87对照组。采用细胞计数试剂盒(CCK-8)分析每组细胞增殖;采用流式细胞术分析每组细胞凋亡水平;采用蛋白质印迹法(Western blot)分析每组细胞Rho超家族成员A(RhoA)和B细胞淋巴瘤/白血病(bcl)-xL表达水平。采用t检验。结果与癌旁组织(1.34±0.21)比较,膀胱癌组织中PCGEM1表达水平(3.91±0.29)显著增加,差异有统计学意义(t=3.109,P<0.05)。与T24对照组和BIU-87对照组(1.80±0.17、1.97±0.21)比较,PCGEM1 KD/T24组和PCGEM1 KD/BIU-87组细胞吸光度值(1.06±0.14、0.96±0.11)显著下降,差异有统计学意义(t=2.701、2.481,P<0.05)。与T24对照组和BIU-87对照组细胞凋亡率[(5.39±1.14)%、(4.90±1.22)%]比较,PCGEM1 KD/T24组和PCGEM1 KD/BIU-87组细胞凋亡率[(31.49±6.12)%、(35.92±5.09)%]显著增加,差异有统计学意义(t=3.081、3.815,P<0.05)。与T24对照组和BIU-87对照组细胞RhoA(1.14±0.15、1.21±0.21)和bcl-xL(0.98±0.11、0.93±0.18)表达水平比较,PCGEM1 KD/T24组和PCGEM1 KD/BIU-87组细胞RhoA(0.31±0.10、0.29±0.09)和bcl-xL (0.38±0.13、0.41±0.11)表达水平显著下降,差异有统计学意义(t=2.517、2.419、2.754,P<0.05)。结论 PCGEM1在膀胱癌中呈高表达,通过调节RhoA和bcl-xL蛋白表达,调节膀胱癌细胞增殖和凋亡。Objective:To observe the effect of prostate cancer gene expression marker 1(PCGEM1)on the proliferation and apoptosis of bladder cancer cells and its molecular mechanism.Methods:159 cases of bladder cancer tissues and adjacent tissues were selected as research objects from June 2016 to June 2019.The expression level of PCGEM1 was analyzed by fluorescence quantitative polymerase chain reaction(PCR).PCGEM1 knockdown and control cell lines were established in bladder cancer cell lines T24 and BIU-87 by RNA interference technology,which were divided into PCGEM1 KD/T24 group,T24 control group,PCGEM1 KD/BIU-87 group and BIU-87 control group,respectively.The cell proliferation of each group was analyzed by cell counting kit-8(CCK-8).The apoptosis level of each group was analyzed by flow cytometry.The expression levels of RhoA and B cell lymphoma/leukemia(bcl)-xL in each group was analyzed by Western blotting.Results:As compared with the adjacent tissues(1.34±0.21),the expression level of PCGEM1 mRNA in the bladder cancer tissues(3.91±0.29)significantly increased(t=3.109,P<0.05).As compared with T24 group and BIU-87 group(1.80±0.17 and 1.97±0.21),PCGEM1 KD/T24 and PCGEM1 KD/BIU-87(1.06±0.14 and 0.96±0.11)significantly decreased(t=2.701 and 2.481,P<0.05).As compared with T24 control group and BIU-87 control group[(5.39±1.14)%and(4.90±±1.22)%],the apoptosis rate of cells in PCGEM1 KD/T24 group and PCGEM1 KD/BIU-87 group[(31.49±6.12)%and(35.92±5.09)%]significantly increased(t=3.081,3.815,P<0.05).As compared with the expression levels of RhoA(1.14±0.15,1.21±0.21)and bcl-xL(0.98±0.11,0.93±0.18)in T24 control group and BIU-87 control group,the expression levels of RhoA(0.31±0.10,0.29±0.09)and bcl-xL(0.38±0.13,0.41±0.11)in PCGEM1 KD/T24 group and PCGEM1 KD/BIU-87 group significantly decreased.Conclusion:PCGEM1 is highly expressed in bladder cancer.PCGEM1 can affect the expression of RhoA and bcl-xL protein and regulate the proliferation and apoptosis of bladder cancer cells.

关 键 词：膀胱癌 前列腺癌基因表达标志物1 Rho超家族成员A 增殖 脱噬作用 

分 类 号：R737.14[医药卫生—肿瘤]