鸡源复合益生菌对青年白羽肉杂鸡免疫球蛋白和Toll样受体通路的影响  

Effect of chicken-derived compound probiotics on immunoglobulin and toll-like receptor pathway of white feather broilers

作　　者：桂雪儿 王志[1] 李思婷 贺濛初 朱杰 冯士彬 吴金节[1] GUI Xueer;WANG Zhi;LI Siting;HE Mengchu;ZHU Jie;FENG Shibin;WU Jinjie(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230061,China;Xuancheng Muzi Poultry Cooperative, Xuancheng 242000, China)

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230061 [2]宣城木子禽业专业合作社,安徽宣城242000

出　　处：《浙江农业学报》2020年第9期1609-1614,共6页Acta Agriculturae Zhejiangensis

基　　金：安徽省重点研究与开发计划(1804a07020135)。

摘　　要：为研究鸡源复合益生菌对青年白羽肉杂鸡血清免疫球蛋白和肠道Toll样受体通路的影响,将180只健康的28日龄白羽肉杂鸡随机分为A(基础饲粮)、B(基础饲粮中添加10^11 CFU·kg^-1益生菌)、C(基础饲粮中添加2×10^11 CFU·kg^-1益生菌)3个处理,每个处理3个重复,每个重复20只鸡。试验期21 d。结果表明:与A组相比,B组和C组鸡血清中IgG、IgM水平显著(P<0.05)升高,B组和C组鸡肠道TLR2、TLR4、Myd88、TRAF-6、AP-1蛋白表达及mRNA相对表达量显著(P<0.05)升高。与B组相比,C组鸡血清IgG、IgM、IgA水平均无明显差异(P>0.05),鸡肠道TLR4、Myd88蛋白表达显著(P<0.05)升高,Myd88基因mRNA相对表达量显著(P<0.05)升高。综上,添加不同浓度的鸡源复合益生菌能够提高白羽肉杂鸡血清中IgG、IgM水平,通过Toll样受体通路增强肉鸡的免疫功能。To investigate the effects of probiotics on serum immunoglobulin and intestinal Toll-like receptor pathway in White feather broiler,one hundred and eighty 28-day-old white feather hybrid broilers were randomly divided into group I(basic diet),group II(basic diet adding 10^11 CFU·kg^-1 probiotics)and group III((basic die adding 2×10^11 CFU·kg^-1 probiotics),with 3 repeats in each group and 20 broilers in each repeat.The experimental period was 21 d.The results showed that,compared with group A,the serum IgG and IgM levels in group B and group C increased significantly(P<0.05).The protein expression of TLR2,TLR4,Myd88,TRAF-6,AP-1 and their relative expression of mRNA in group B and group C were significantly(P<0.05)increased.Compared with group B,the serum IgG,IgM and IgA levels in group C were no significant difference(P>0.05).The protein expression of TLR4 and MyD88 in group C were significantly(P<0.05)increased,and the relative expression of MyD88 mRNA was significantly(P<0.05)increased.Feeding probiotics can improve the serum IgG and IgM levels of white feather broilers,and regulate the immune function of broiler intestine effectively through toll like receptor pathway.

关 键 词：乳酸菌 枯草芽孢杆菌 白羽肉杂鸡 免疫球蛋白 Toll样受体通路 

分 类 号：S831[农业科学—畜牧学]