针刺腧穴“降压方”对自发性高血压大鼠心肌氧化应激的影响  

Effects of Acupoints Prescription“Jiangyafang”on Myocardial Oxidative Stress in Spontaneously Hyper⁃tensive Rats

作　　者：刘高峰 郭继龙 冀雨芳 董爱爱 来晓云 李赟 谢宽 高珍 冀来喜 Liu Gaofeng;Guo Jilong;Ji Yufang;Dong Aiai;Lai Xiaoyun;Li Yun;Xie Kuan;Gao Zhen;Ji Laixi(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学,天津301617 [2]山西中医药大学,山西晋中030619 [3]成都中医药大学,四川成都611137

出　　处：《中国中医急症》2020年第9期1519-1523,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81673887)。

摘　　要：目的探讨针刺腧穴"降压方"对自发性高血压大鼠(SHR)心肌氧化应激的影响。方法将30只雄性SHR随机分为模型组(SHR)、假针刺组(SHR+Sham)和降压方组(SHR+Acu)各10只,10只Wistar Kyoto大鼠作为正常组(WKY)。SHR+Acu组选取双侧人迎穴、曲池穴、足三里穴,每周连续治疗6 d,休息1 d;SHR+Sham组浅刺各穴,不行针;SHR组与WKY组仅束缚处理,4组大鼠均干预8周。选用无创血压仪在干预前及干预第2、4、6、8周末测量4组大鼠尾动脉收缩压;选用DHE染色法观察各组大鼠心肌活性氧(ROS)含量,生化检测大鼠心肌超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)含量,RT-qPCR检测SHR心肌非吞噬细胞氧化酶2(NOX2)、非吞噬细胞氧化酶4(NOX4)m RNA的表达水平。结果长期针刺人迎穴、曲池穴、足三里穴组成的腧穴"降压方"可以显著降低SHR的血压,抑制心肌ROS生成,降低NOX4 mRNA表达,提高SOD活性(P<0.05);同时,GSH-PX活性也有所提高,NOX2 m RNA的表达也有下降趋势(P<0.05)。而假针刺治疗无此作用(P>0.05)。结论针刺腧穴"降压方"可显著降低SHR血压,提高心肌的抗氧化应激能力以减轻心肌损伤,其机制可能与其抑制心肌ROS生成,提高心肌SOD、GSH-PX活性,提升清除氧自由基的能力,抑制心肌NOX2、NOX4 mRNA的表达有关。Objective:To investigate the effect of acupoints prescription“Jiangyafang”on myocardial oxidative stress in spontaneously hypertensive rats(SHR).Methods:A total of 30 male SHR were randomly divided into model group(SHR),sham acupuncture group(SHR+Sham)and"Jiangyafang"group(SHR+Acu)with 10 Wistar Kyoto rats as normal group(WKY).In the SHR+Acu group,bilateral Renying,Quchi and Zusanli were selected for continuous treatment for 6 d per week and rest for 1 d.SHR+Sham group shallowly pricked all the points without needles.The SHR group and the WKY group were only bound,and the rats in the 4 groups were all interfered for 8 weeks.Before the intervention,noninvasive blood pressure instrument was used to measure the systolic pressure of tail artery in 4 groups of rats at the end of 2,4,6 and 8 weeks after the intervention.DHE staining was used to observe the contents of reactive oxygen species(ROS)in rat myocardium in each group,the contents of SOD and GSH-PX in rat myocardium were determined,and the mRNA expression levels of oxidase 2(NOX2)and oxidase 4(NOX4)in SHR myocardium were detected by RT-qPCR.Results:Long-term acupuncture of“Jiangyafang”at the acupoints including Renyin,Quchi and Zusanli could significantly reduce SHR's blood pressure,inhibit the production of myocardial ROS,reduce NOX4 mRNA expression and increase SOD activity(P<0.05).Meanwhile,the activity of GSH-PX was also increased,and the expression of NOX2 mRNA also showed a downward trend(P<0.05).However,sham acupuncture treatment had no such effect(P>0.05).Conclusion:Acupoints prescription“Jiangyafang”can significantly reduce SHR blood pressure,improve the ability of myocardial anti-oxidative stress to reduce myocardial injury,the mechanism may be related to the inhibition of myocardial ROS production,in⁃crease the activity of myocardial SOD,GSH-PX,enhance the ability of scavenging oxygen free radicals,and inhibit the expression of myocardial NOX2,NOX4 mRNA.

关 键 词：高血压 针刺 腧穴“降压方” SHR 心肌 ROS 氧化应激 

分 类 号：R245[医药卫生—针灸推拿学]