三维组织学重建技术研究小鼠睾丸的下降过程  

Application of three dimensional histological reconstruction technology research testicular descent on mice

作　　者：巫海锋 谢肖俊 李建宏[3] 张镟 蒋学武 Wu Haifeng;Xie Xiaojun;Li Jianhong;Zhang Xuan;Jiang Xuewu(Department of Pediatric Surgery,Jiangmen City Center Hospital,Jiangmen 529000,Guangdong Province,China;Department of Pediatric Surgery,the First Affiliated Hospital,Medical College of Shantou University,Shantou 515041,Guangdong Province,China;Department of Pediatric Surgery,the Second Affiliated Hospital,Medical College of Shantou University,Shantou 515041,Guangdong Province,China;Department of Pediatric Surgery,Woman&Children′s Hospital,Shenzhen University and Pingshan District,Shenzhen 518122,Guangdong Province,China)

机构地区：[1]江门市中心医院小儿外科,广东江门529000 [2]汕头大学医学院第一附属医院小儿外科,广东汕头515041 [3]汕头大学医学院第二附属医院小儿外科,广东汕头515041 [4]深圳市坪山区妇幼保健院暨深圳大学妇幼保健院小儿外科,广东深圳518122

出　　处：《中华实用儿科临床杂志》2020年第17期1320-1324,共5页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(81501240);深圳市科创委科研项目(JCYJ20150402092905161)。

摘　　要：目的应用三维(3D)组织学重建技术观察研究小鼠睾丸下降过程及其毗邻组织器官形态、位置的时序性变化。方法取怀孕(GD)及出生后(PD)不同天数(GD15、GD17、GD19及PD3、PD7)雄性昆明小鼠肾脏平面以下整块组织连续切片、HE染色,再使用PerkinElmer全自动切片分析系统进行镜下图像全景扫描,Photoshop软件配准、对齐,3D-doctor软件进行3D重建及分析。结果1.附睾将睾丸包绕其中,附睾尾部与睾丸引带相连,睾丸下极与睾丸引带未见有直接连接;孕期时,小鼠附睾各部分及输精管相互堆积成团,不易于辨认,睾丸引带发生"膨大反应",出生后,附睾变大、拉长,在形态上可辨认,睾丸引带则退化成纤维索带样组织,在整个过程中,睾丸引带的体积在形态上明显小于睾丸的体积。2.双侧睾丸形态相近,位置不对称,左侧睾丸位置低于右侧,并先于右侧开始下降;孕期时,小鼠双侧睾丸从肾下极开始下降,左侧睾丸下降至膀胱下部位置,右侧睾丸下降至膀胱中部位置,出生后,左侧睾丸降至膀胱颈部以下,右侧睾丸降至膀胱颈部,最终是左侧睾丸位置低于右侧。3.附睾先于睾丸启动下降;在整个过程中,附睾尾部始终先于睾丸开始下降,附睾尾下降后拉动整个附睾下降,睾丸则沿着附睾体的下降路径进入阴囊,下降完全后,附睾尾始终位于睾丸之下。结论小鼠睾丸的下降过程可能是睾丸引带首先牵引附睾尾部下降至内环口,附睾尾部及体部通过其形态改变不断扩张内环口及腹股沟管,并继续降入阴囊且扩大阴囊,睾丸及附睾头顺着附睾体通过扩大的内环、腹股沟管进入阴囊。Objective To explore the testicular descent process and temporal morphology and position changes of its neighboring tissues and organs in mice with three dimensional(3D)histological reconstruction technique.Methods Tissue below the kidney plane of male Kunming mice was harvested on gestation days(GD)15,GD17,GD19,postnatal days(PD)3 and PD7.The tissue was then serially sectioned and stained with hematoxylin and eosin(HE).Slices were scanned and saved by the PerkinElmer automatic section analysis system.Afterward,Photoshop software was used for image registration and alignment,and 3D-doctor software was employed for 3D reconstruction and analysis.Results(1)The epididymis enveloped the testis,and the tail of the epididymis was connected with the gubernaculum,but no direct connection was found between the lower pole of the testis and the gubernaculum.During pregnancy,each part of the epididymis and the vas deferens of mice were piled up into a mass,making it hard to identify the epididymis.The gubernaculum leads to"swelled".After birth,the epididymis became large,elongated,and morphologi-cally recognizable,and the gubernaculum degenerated into fibrous cords.During the whole process,the volume of gubernaculum was significantly smaller than that of testicle.(2)Testicles on both sides were similar in shape and asymmetrical in position.The left testicle was lower than the right testicle,and descended before the right one.During pregnancy,testicles on both sides descended from the lower pole of the kidney,the left testical stopped at the lower part of the bladder.and the right one stopped at middle part of the bladder.After birth,the left testicle dropped below the neck of the bladder.Finally,the left testicle was lower than the right testicle.(3)The epididymis descend prior to the testicles.Throughout the process,the tail of the epididymis descended before the testicles,and it pulled the whole epididymis down.Testicles entered the scrotum along the descending path of the epididymis.After the complete descent,the tail of

关 键 词：形态学 三维组织学重建 睾丸下降 附睾下降 小鼠 

分 类 号：R-332[医药卫生] R726.9