TMPRSS4在裸鼠结肠癌肝转移模型的促癌机制研究  

Carcinogenic mechanism research of TMPRSS4 in nu-/nu-mice model of colon cancer liver metastasis

作　　者：于登峰[1] 杨宇慎 王艺[1] YU Deng-feng;YANG Yu-shen;WANG Yi(Department of General Surgery,the Affiliated Xinhua Hospital of Dalian University,Dalian 116021,Liaoning,CHINA)

机构地区：[1]大连大学附属新华医院普通外科,辽宁大连116021

出　　处：《海南医学》2020年第18期2313-2317,共5页Hainan Medical Journal

摘　　要：目的检测TMPRSS4在裸鼠结肠癌肝转移模型中的表达,并探究其促癌机制。方法采用转染基因重组质粒pcDNA3.1(-)/TMPRSS4和pcDNA3.1(-)/Scramble的HT29结肠癌细胞株建立20只裸鼠肝转移模型,并按随机数表法分成TMPRSS4组(研究组,10只)和Scramble组(对照组,10只)。制模6周后处死观察两组裸鼠肝脏的组织形态学和组织病理学改变情况,并将其标本采用RT-PCR和Western blot检测裸鼠结肠癌肝转移模型中的TMPRSS4表达情况。结果成功构建稳定型表达TMPRSS4和Scramble重组载体的HT29细胞株,并100%(20/20)成功完成两组裸鼠结肠癌肝转移模型;从组织标本的形态学和病理学角度分析,与对照组相比,研究组裸鼠结肠癌肝转移评分[(2.400±0.516)分vs(3.700±0.483)分]显著增高,差异有统计学意义(P<0.05);从组织标本的分子生物学角度分析,与对照组相比,研究组裸鼠的mRNA[(1.060±0.207)vs(2.280±0.712)]和蛋白质[(0.740±0.114)vs(2.580±0.719)]表达显著增高,差异均有统计学意义(P<0.05)。结论TMPRSS4可有效促进裸鼠结肠癌肝转移的进程,至于具体的促转移机制则有待于进一步的研究揭示。Objective To test the expression of transmembrane protease/serine 4(TMPRSS4)and investigate it’s carcinogenic mechanism in nu-/nu-mice model of colon cancer liver metastasis.Methods HT29 colon cancer cells transfected by recombinant vector pcDNA3.1(-)/TMPRSS4 or pcDNA3.1(-)/Scramble of vector were used to establish a stable hepatic metastasis of colon cancer nu-/nu-mice model.Twenty of nu-/nu-mice were divided into TMPRSS4 study group(n=10)and Scramble control group(n=10)by random number table.Six weeks later,histomorphological and histopathological changes of liver metastases were tested,meanwhile,RT-PCR and Western blot were used to detect the expression of TMPRSS4 in both groups.Results HT29 colon cancer cells steadily expressing TMPRSS4 and Scramble recombinant vector were successfully built,and the tumor formation rate was 100%(20/20)in two groups.For the histomorphological and histopathological analysis,the liver metastasis scores in TMPRSS4 study group were 3.700±0.483,which were significantly higher than 2.400±0.516 in the Scramble control group(P<0.05).For the analysis of molecular biology,compared with the control group,the expressive level of mRNA and protein were markedly higher those in the study group(P<0.05):(1.060±0.207)vs(2.280±0.712);(0.740±0.114)vs(2.580±0.719).Conclusion TMPRSS4 may effectively facilitate the progression of colon cancer liver metastasis in nu-/nu-mice model,but further studies are needed to clarify the specific metastasis mechanism.

关 键 词：TMPRSS4 裸鼠 结直肠癌肝转移 促癌机制 促转移作用 

分 类 号：R-332[医药卫生]