苦参碱调控Sirt1/FoxO3信号通路促进胃癌细胞SGC7901凋亡的机制研究  

The effects and its action mechanism of matrine in promoting cell apoptosis of gastric cancer SGC7901 cells by regulating Sirt1/FoxO3 signaling pathway in vitro

作　　者：熊颖[1] 郭芳 熊宇 张万里 胡艳艳 XIONG Ying;GUO Fang;XIONG Yu(Department of Gastroenterology,Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Hubei, Wuhan 430022, China)

机构地区：[1]华中科技大学同济医学院附属协和医院消化内科,武汉市430022 [2]华中科技大学同济医学院附属协和医院胃肠外科,武汉市430022 [3]湖北文理学院附属医院湖北省襄阳市中心医院消化内科

出　　处：《河北医药》2020年第19期2900-2904,共5页Hebei Medical Journal

基　　金：湖北省自然科学基金项目(编号:WJ2015MB075)。

摘　　要：目的研究苦参碱促进胃癌细胞SGC7901细胞凋亡的AKT/FoxO3信号通路机制,为临床新药研发提供参考。方法将生长状态良好的胃癌SGC7901细胞随机分为对照组、Nicotinamide处理组(20μmol/L)、苦参碱低剂量处理组(10μmol/L)、苦参碱高剂量处理组(40μmol/L)。Western Blotting法分析细胞凋亡蛋白Caspase-3/9、Bax/Bcl-2及Sirt1/FoxO3蛋白的表达,DAPI染色分析各组细胞的凋亡情况;免疫细胞化学法分析Sirt1/FoxO3蛋白的表达情况。结果与对照组比较,Nicotinamide组和苦参碱组细胞Bax、Caspase-3/9和FoxO3的表达明显增强(P<0.05),而Bcl-2、Sirt1蛋白的表达明显减弱(P<0.05),DAPI染色凋亡小体明显增多;免疫组织化学发现FoxO3的荧光强度增强(P<0.05),而Sirt1蛋白的荧光强度减弱(P<0.05),且苦参碱的效果有剂量依从性(P<0.05)。结论苦参碱可能通过调控Sirt1/FoxO3信号通路促进胃癌细胞SGC7901的细胞凋亡过程。Objective To investigate the effects and its action mechanism of matrine in promoting cell apoptosis of gastric cancer SGC7901 cells by regulating Sirt1/FoxO3 signaling pathway in vitro,so as to provide reference for research and development of clinical new drugs.Methods Gastric cancer cell line SGC7901 cells were randomly divided into 4 groups:control group,nicotinamide group(20μmol/L),low dose matrine group(10μmol/L)and high dose matrine group(40μmol/L).The expression levels of Caspase-3/9,Bax/Bcl-2 and Sirt1/FoxO3 were detected by Western Blot.The cell apoptosis status was assayed by DAPI staining.Moreover,the expression levels of Sirt1/FoxO3 signaling pathway related proteins were detected by immunocytochemistry.Results As compared with those in control group,the expression levels of Caspase-3/9 and Bax were significantly increased in nicotinamide group and in matrine groups,however,the expression levels of Bcl-2 and Sirt1/FoxO3 were significantly decreased greatly(P<0.05).Moreover the apoptotic body and fluorescence intensity of FoxO3 were significantly increased in nicotinamide group and matrine groups,but the fluorescence intensity of Sirt1 protein was significantly decreased,and the effects of matrine showed a dose dependent manner(P<0.05).Conclusion The matrine can promote the cell apoptosis of gastric cancer SGC7901 cells by regulating Sirt1/FoxO3 signaling pathway in vitro.

关 键 词：苦参碱 细胞凋亡 DAPI染色 免疫细胞化学 Sirt1/FoxO3信号通路 

分 类 号：R735.2[医药卫生—肿瘤]