浙江省马铃薯病毒病检测分析  

Detection and analysis of potato viruses in Zhejiang province

作　　者：陈莹 田艳珍 王芳 胡淑珍 陈步扬 彭杰军 鲁宇文 燕飞 陈剑平 CHEN Ying;TIAN Yanzhen;WANG Fang;HU Shuzhen;CHEN Buyang;PENG Jiejun;LU Yuwen;YAN Fei;CHEN Jianping(College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China;College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;Institute of Biotechnology, Ningbo Academy of Agricultural Sciences, Ningbo 315052, China;College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;Institute of Plant Virology, Ningbo University, Ningbo 315211, China)

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866 [2]福建农林大学植物保护学院,福州350002 [3]宁波市农业科学院生物技术研究所,宁波315052 [4]南京农业大学植物保护学院,南京210095 [5]宁波大学植物病毒学研究所,宁波315211

出　　处：《植物保护》2020年第5期110-115,121,共7页Plant Protection

基　　金：国家重点研发计划(2018YFD0200802)。

摘　　要：马铃薯病毒病是影响马铃薯产量和品质的主要因素之一,其症状表现为花叶、黄化和卷曲等。2019年4月-5月,在浙江省湖州、杭州、绍兴、宁波、金华、台州和丽水等7个地市的主要马铃薯产区采集了具有典型病毒病症状的马铃薯样品,采用转录组测序和RT-PCR验证的方法进行病毒检测,共筛查并验证出6种马铃薯病毒,包括马铃薯X病毒Potato virus X(PVX)、马铃薯Y病毒Potato virus Y(PVY)、马铃薯S病毒Potato virus S(PVS)、马铃薯H病毒Potato virus H(PVH)、马铃薯M病毒Potato virus M(PVM)以及马铃薯奥古巴花叶病毒Potato aucuba mosaic virus(PAMV)。检测结果表明,在调查的这7个地市中每个地市都具有2种以上的病毒发生,其中PVH作为一种新鉴定的麝香石竹潜隐病毒属成员在除宁波以外的各地区都有发生;此外杭州市、湖州市以及绍兴市均检测出了PAMV。对检测到的PVS、PVH、PVM和PAMV外壳蛋白(coat protein,CP)序列进行系统发育分析表明,这些病毒均存在地域差异性。本研究为浙江省马铃薯病毒的防控奠定了重要基础。Potato virus disease,with symptoms of mosaic,yellowing and curling,is one of the main factors affecting potato yield and quality.During April-May 2019,we collected samples with typical symptoms in the main potato-producing areas in seven cities including Huzhou,Hangzhou,Shaoxing,Ningbo,Jinhua,Taizhou and Lishui.A comprehensive and accurate detection was conducted by applying the transcriptome sequencing and the RT-PCR verification.A total of 6 potato viruses were screened out,including Potato virus X(PVX),Potato virus Y(PVY),Potato virus S(PVS),Potato virus H(PVH),Potato virus M(PVM),and Potato aucuba mosaic virus(PAMV).The results showed that multiple viruses,two or more viruses,occurred in all seven cities.PVH,as a newly identified member of the genus Carlavirus,occurred in all regions except Ningbo.In addition,PAMV,first reported in 2018 in China,was detected in Hangzhou,Huzhou and Shaoxing cities.Phylogenetic analysis was performed based on coat protein(CP)sequences of PVS,PVH,PVM and PAMV.This study laid an important foundation for the prevention and control of potato viruses in Zhejiang province.

关 键 词：马铃薯 病毒检测 马铃薯H病毒 马铃薯奥古巴花叶病毒 系统发育分析 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]