NOD/SCID小鼠皮下及肾包膜下子宫内膜癌移植瘤模型的实验研究  

Research of subrenal and subcutaneous transplantation of endometrial cancer cells in a NOD/SCID mouse xenograft model

作　　者：黄燕[1] 江明 杨晓清[1] 张磊[3] 任萍[1] 杨颖莉[1] 张玉泉[1] HUANG Yan;JIANG Ming;YANG Xiaoqing;ZHANG Lei;REN Ping;YANG Yingli;ZHANG Yuquan(Department of Gynecology and Obstetrics,the Affiliated Hospital of Nantong University,Nantong 226001;Laboratory of Nuclear Receptors and Cancer Research,Medical School of Nantong University;Department of Ultrasound,the Affiliated Hospital of Nantong University)

机构地区：[1]南通大学附属医院妇产科,南通226001 [2]南通大学医学院核受体与肿瘤研究实验室 [3]南通大学附属医院超声科

出　　处：《南通大学学报（医学版）》2020年第4期305-310,共6页Journal of Nantong University(Medical sciences)

基　　金：国家国际科技部合作项目(2011RR0001)。

摘　　要：目的:采用人子宫内膜癌细胞株建立非肥胖糖尿病/严重联合免疫缺陷(non-obese diabetic/severe combined immunodeficient,NOD/SCID)小鼠肾包膜下及皮下异种移植模型,为进一步研究其肿瘤学特性提供良好的动物模型。方法:绿色荧光蛋白转染Ishikawa细胞和HEC-1-A细胞,将转染后的细胞以一定浓度(1×10^5、2×10^5、4×10^5)种植入NOD/SCID小鼠体内,观察8周后小鼠的成瘤情况。结果:移植瘤生长具有剂量依赖效应,Ishikawa皮下组模型中,细胞浓度为1×10^5时,成瘤率为33.3%,当细胞浓度为2×10^5和4×10^5时,成瘤率均为66.7%。将1×10^5个细胞接种于肾包膜下,8周后处死小鼠时未见肿瘤形成,而细胞浓度调整为2×10^5时,成瘤率为33.3%,细胞浓度为4×10^5时,成瘤率为50.0%。使用HEC-1-A建立皮下及肾包膜下动物模型,其成瘤率均为100%,且肾包膜组明显优于皮下组(P=0.047),4×10^5是最适合的建模浓度。结论:采用HEC-1-A建立人子宫内膜癌动物模型时,肾包膜比皮下建模更具有优势。采用Ishikawa建立人子宫内膜癌动物模型时,在本文特定的细胞浓度相比,皮下建模似乎更合适。Objective:At present,there have been few reports regarding the use of non-obese diabetic/severe combined immunodeficient(NOD/SCID)mice to prepare endometrial cancer xenograft model systems.Herein,we therefore sought to develop such a model in an effort to better evaluate endometrial cancer cell line tumorigenicity.Methods:We compared the susceptibilities of endometrial cancer cell lines for tumor formation when transplanted as subcutaneous and subrenal capsule xenografts.Various numbers of Ishikawa and HEC-1-A cells(1×10^5,2×10^5,4×10^5)transfected with green fluorescent protein were inoculated into NOD/SCID mice and tumor sizes were measured after 8 weeks.Results:Tumors were formed reproducibly and in a dose-dependent manner.Subcutaneous inoculation of Ishikawa cells resulted in tumor formation was 33.3%with 1×10^5 cells,and inoculated with 2×10^5 or 4×10^5 cells,the tumor formation rate was 66.7%respectively.In the subrenal group,inoculation of 1×10^5 Ishikawa cells resulted in no tumor formation,while inoculated with 2×10^5 cells,the tumor formation rate was 33.3%,and tumors developed 50.0%inoculated with 4×10^5 cells.For HEC-1-A cells,the rates of successful subrenal and subcutaneous endometrial cell xenograft transplantations were both 100%.Subrenal xenografts were superior to subcutaneous xenografts for HEC-1-A cells(P=0.047).Final tumor volume increased significantly with increasing number of HEC-1-A cells inoculated,and 4×10^5 was thus identified as the ideal inoculation concentration for subrenal xenografts.Conclusion:HEC-1-A cell xenografts form tumors more readily after subrenal than subcutaneous inoculation.However,subcutaneous inoculation may represent a good choice in the event of limited numbers of Ishikawa cells.

关 键 词：非肥胖糖尿病/严重联合免疫缺陷 皮下模型 肾包膜下模型 子宫内膜癌 小鼠 

分 类 号：R737.33[医药卫生—肿瘤]