TREM-1在炎症性肠病患者血清中的表达及其与疾病活动度的关系  

Expression of serum myeloid cell trigger receptor 1 in patients with inflammatory bowel disease and its relationship with disease activity

作　　者：王利民[1] 沈利洪 马闪珊[1] 张丽英[1] 吴俊琪[1] WANG Limin;SHEN Lihong;MA Shanshan;ZHANG Liying;WU Junqi(Department of Clinical Laboratory,Jinhua Central Hospital,Jinhua 321000,China)

机构地区：[1]金华市中心医院检验科,321000

出　　处：《浙江医学》2020年第18期1968-1972,I0006,共6页Zhejiang Medical Journal

基　　金：金华市科学技术研究计划公益类项目(2018-4-042)。

摘　　要：目的探讨血清髓系细胞触发受体1(TREM-1)在炎症性肠病(IBD)患者血清中的表达及其与疾病活动度的关系。方法选取2018年6月至2020年2月金华市中心医院收治的IBD患者169例,其中溃疡性结肠炎(UC)78例、克罗恩病(CD)91例。另择同期收治的普通腹泻患者40例,健康体检者40例。采用ELISA法检测受试者TREM-1水平与血钙卫蛋白(CALP)、WBC、ESR、CRP水平。比较IBD患者、普通腹泻患者、健康体检者血清TREM-1水平;分析IBD患者血清TREM-1水平与血CALP、WBC、ESR、CRP水平的相关性;分析TREM-1对UC活动期和CD活动期的诊断效能。结果UC活动期、CD活动期TREM-1水平高于普通腹泻患者和健康体检者(均P<0.05)。UC缓解期、CD缓解期TREM-1水平与普通腹泻患者和健康体检者比较差异无统计学意义(P>0.05)。UC活动期与缓解期、CD活动期与缓解期的TREM-1水平比较差异有统计学意义(均P<0.05),活动期高于缓解期。UC患者TREM-1水平与CALP、WBC、ESR、CRP水平均呈正相关(r=0.972、0.362、0.371、0.297,均P<0.05);CD患者TREM-1水平与CALP、WBC水平呈正相关(r=0.922、0.608,均P<0.05),与CRP、ESR无关(均P>0.05)。CD患者TREM-1水平51.241 pg/ml时,AUC为0.706,诊断活动期的灵敏度为0.615,特异度为0.798。UC患者TREM-1水平为83.091 pg/ml时,AUC为0.878,诊断活动期的灵敏度为0.738,特异度为0.972。结论IBD患者血清TREM-1表达水平上调,对其疾病活动期有较高的诊断效能。Objective To explore the expression of triggering receptor expressed on myeloid cells 1(TREM-1)in patients with inflammatory bowel disease(IBD)and its relationship with disease activity.Methods A total of 169 IBD patients treated at Jinhua Central Hospital from June 2018 to February 2020 were enrolled,including 78 cases of ulcerative colitis(UC)and 91 cases of Crohn's disease(CD).Forty patients with common diarrhea and 40 healthy subjects served as controls.The serum trem-1 levels were detected by ELISA.The Calprotectin(CALP),leukocyte(WBC),erythrocyte sedimentation rate(ESR)and c-reactive protein(CRP)were also measured in all subjects.The correlation of serum TREM-1 level with CALP,WBC,ESR and CRP levels in IBD patients was analyzed,and the diagnostic efficacy of TREM-1 during UC and CD activity period was analyzed.Results The serum trem-1 levels in the active stage of UC and CD were higher than those in the common diarrhea patients and healthy subjects(P<0.05).The level of trem-1 was no significant difference between the remission stage of UC and CD disease(P>0.05).In both UC and CD disease,compared with the remission stage,the level of trem-1 in the active stage was higher(both P<0.05).In the UC group,the trem-1 level was positively correlated with CALP,WBC,ESR and CRP(r=0.972,0.362,0.371,0.297,all P<0.05).In the CD group,the trem-1 level was positively correlated with CALP,WBC(r=0.922,0.608,P<0.05).However,the trem-1 level was no correlation compared with CRP and ESR(P>0.05).The area under the curve of trem-1 for diagnosis of active CD was 0.706.And taking 51.241pg/ml as cut-off value the sensitivity and specificity were 61.5%and 79.8%respectively.The area under the curve of trem-1 for diagnosis of active UC was 0.878,and taking 83.091 pg/ml as cut-off value,the sensitivity and specificity were 73.8%and 97.2%respectively.Conclusion The expression level of serum TREM-1 in IBD patients was increased,and the diagnosis efficacy was higher during the period of disease activity.

关 键 词：髓系细胞触发受体1 炎症性肠病 受试者工作特征曲线 

分 类 号：R574[医药卫生—消化系统]