“汗渍法”炮制对了哥王中芫花素含量及其抗氧化能力的影响  

Effects of “Sweat Soaking Method” on the Content of Genkwanin in Wikstroemia indica and Its Antioxidation Ability

作　　者：周志容 冯果 李玮 郑传奇 许琴 任晨晨 彭礼珍 黄侨宗 肖晓燕 ZHOU Zhirong;FENG Guo;LI Wei;ZHENG Chuanqi;XU Qin;REN Chenchen;PENG Lizhen;HUANG Qiaozong;XIAO Xiaoyan(College of Pharmacy,Guizhou University of TCM,Guiyang 550025,China;National Engineering and Technology Research Center of Miao Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025 [2]国家苗药工程技术研究中心,贵阳550025

出　　处：《中国药房》2020年第19期2320-2325,共6页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81760766);国家中医药管理局中药炮制技术传承基地建设项目(No.国中医科技中药便函〔2015〕132号);全国中医药创新骨干人才培训项目(No.国中医药人教函〔2019〕128号);贵州省科技计划项目(No.黔科合基础〔2017〕1007);贵州省高层次创新型人才项目(No.黔人领发〔2020〕4号);贵州中医药大学博士启动基金项目(No.贵中医博士基金〔2017〕1号)。

摘　　要：目的:比较"汗渍法"炮制前后了哥王乙醇提取物中活性/毒性成分芫花素的含量变化,以及炮制对其抗氧化能力的影响。方法:采用高效液相色谱法测定"汗渍法"炮制前后了哥王药材中芫花素的含量。色谱柱为Diamonsil C18,流动相为0.2%磷酸水溶液-甲醇(梯度洗脱),流速为1 mL/min,柱温为30℃,检测波长为346 nm,进样量为20μL。将SD大鼠随机分为空白组、了哥王生品乙醇提取物组(317.52 mg/kg,简称"生品组")和了哥王炮制品乙醇提取物组(317.52 mg/kg,简称"炮制品组"),每组6只。空白组大鼠灌胃等体积1.0%羧甲基纤维素钠溶液,各给药组灌胃相应药物混悬液,灌胃体积均为20 mL/kg,每日1次,连续14 d。采用酶联免疫吸附测定法检测各组大鼠血清氧化应激指标[丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)]的含量。结果:芫花素进样检测量的线性范围为0.147~27.360μg(r=0.999 9);精密度、重复性、稳定性试验的RSD均小于3%;平均加样回收率为98.64%~98.92%(RSD<1%,n=3)。"汗渍法"炮制前后,了哥王药材中芫花素的平均含量分别为0.377 6、0.234 0mg/g。与空白组比较,生品组大鼠血清中SOD含量显著升高,CAT含量显著降低(P<0.05或P<0.01);炮制品组大鼠血清中MDA含量显著降低,SOD含量显著升高(P<0.05或P<0.01);且炮制品组MDA含量显著低于生品组,SOD含量显著高于生品组(P<0.05)。结论:经"汗渍法"炮制后,了哥王药材中芫花素的含量有所降低,且抗氧化活性增强;"汗渍法"具有一定的"减毒增效"效果。OBJECTIVE:To compare the content changes of active/toxic ingredient genkwanin in ethanol extract from Wikstroemia indica before and after processing with"sweat soaking method"and the effects of processing method on its antioxidation ability.METHODS:HPLC method was adopted to determine the content of genkwanin in W.indica before and after processing with"sweat soaking method".The separation was performed on Diamonsil C18 column with 0.2%phosphoric acid solution-methanol as mobile phase(gradient elution)at the flow rate of 1 mL/min.The column temperature was 30℃and detection wavelength was set at 346 nm.The sample size was 20μL.SD rats were randomly divided into blank group,W.indica raw product ethanol extract group(317.52 mg/kg,called"raw-product group"as short)and W.indica processed product ethanol extract group(317.52 mg/kg,called"processed-product group"as short),with 6 rats in each group.Blank group was given constant volume of 1.0%CMC-Na solution intragastrically,and administration groups were given relevant medicine suspension intragastrically;all of them were given 20 mL/kg,once a day,for consecutive 14 days.The contents of serum oxidant stress indexes(MDA,CAT,SOD)in rats were determined by ELISA.RESULTS:The linear range of genkwanin were 0.147-27.360μg(r=0.9999);RSDs of precision,reproducibility and stability tests were all lower than 3%;average recoveries were 98.64%-98.92%(RSD<1%,n=3).Before and after processing with"sweat soaking method",average contents of genkwanin in W.indica were 0.3776 and 0.2340 mg/g.Compared with blank group,the serum content of SOD in raw-product group was increased significantly,while CAT content was decreased significantly(P<0.05 or P<0.01);the serum content of MDA was decreased significantly in processed-product group,while SOD content was increased significantly(P<0.05 or P<0.01).MDA content of processed-product group was significantly lower than that of raw-product group,while SOD content was significantly higher than raw-product group(P<0.05).CONCLUSIONS:After processi

关 键 词：了哥王 汗渍法 芫花素 含量 抗氧化能力 减毒增效 

分 类 号：R283.1[医药卫生—中药学] R285[医药卫生—中医学]