脱氢/还原酶及其辅酶再生循环的研究进展  

Progress on dehydrogenase/reductase and coenzyme regeneration

作　　者：杜美妮 许超[1] 黄金[1] DU Meini;XU Chao;HUANG Jin(College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江工业大学药学院,浙江杭州310014

出　　处：《发酵科技通讯》2020年第3期147-152,共6页Bulletin of Fermentation Science and Technology

摘　　要：脱氢/还原酶因具有高度的区域和立体选择性,常用于生物催化合成在化学、医药和精细化学品等领域有重要应用价值的手性化合物。介导双酮基还原的脱氢/还原酶是特殊的羰基还原酶,在辅酶NAD(P)H的存在下,可催化二酮类底物得到手性双醇。分析了国内外关于介导双酮基还原的脱氢/还原酶酶偶联和底物偶联法辅酶再生的研究进展,提出了辅酶再生循环系统的理性改造策略。Dehydrogenase/reductase is commonly used in biosynthesis of chiral compounds in the fields of chemistry,medicine and fine chemicals due to its high regioselectivity and stereoselectivity.In the presence of the coenzyme NAD(P)H,dehydrogenases/reductases that can mediate diketone reduction are special carbonyl reductases.In this paper,from the perspective of the coenzyme regeneration,we have analyzed the domestic and international research progress on dehydrogenase/reductase which mediates diketone reduction with enzyme-coupled and substrate-coupled methods.Finally,we proposed a rational transformation strategy for the coenzyme-regeneration cycle system.

关 键 词：脱氢/还原酶 双酮基还原 辅酶再生 酶偶联 底物偶联 

分 类 号：TQ464.8[化学工程—制药化工]