超声提取-离子色谱-脉冲积分安培检测法检测黑果腺肋花楸果中的8种单糖和双糖  

Determination of 8 kinds of monosaccharides and disaccharides in Aornial mealnocarpa fruit by ultrasonic extraction-ion chromatography-pulse integral ampere detection

作　　者：于丽 刘瑜 周健南 裴程程 YU Li;LIU Yu;ZHOU Jian-Nan;PEI Cheng-Cheng(Shenyang Customs,Shenyang 110016,China)

机构地区：[1]沈阳海关,沈阳110016

出　　处：《食品安全质量检测学报》2020年第17期6104-6109,共6页Journal of Food Safety and Quality

基　　金：海关总署科研项目(2019HK105)。

摘　　要：目的建立超声提取-离子色谱-脉冲积分安培检测法测定黑果腺肋花楸果中的8种单糖和双糖的方法。方法采用水浸提超声提取及膜过滤法前处理黑果腺肋花楸果样品,以Dionex CarboPac PA10 Analytical Column(4 mm×250 mm)阴离子交换色谱柱为分析柱,以Dionex CarboPac PA10 Guard Column(4 mm×50 mm)为保护柱,以NaOH与CH3COONa的组合溶液作为流动相,采用梯度淋洗分离程序,脉冲积分安培方法检测。结果8种单糖和双糖的检出限分别为5、8、3、4、7、2、5、2μg/L,且均具有较宽的线性范围(0.05~20 mg/L)。黑果腺肋花楸果中单糖和双糖测定的相对标准偏差在0.96%~6.12%之间,8种单糖和双糖的加标回收率达到90.5%~102.1%。结论本方法同时检测黑果腺肋花楸果中的单糖和双糖,其方法简便快捷、分离效果好、基体干扰少、无需衍生、灵敏度高,适用于黑果腺肋花楸果中的常见单糖和双糖组分的分析。Objective To establish a method for the determination of eight kinds of monosaccharides and disaccharides in Aornial mealnocarpa by ultrasonic extraction,ion chromatography-pulse-integral amperometric detection.Methods The samples of Aornial mealnocarpa fruit were pretreated by ultrasonic extraction with water extraction and membrane filtration.The analytical column was Dionex CarboPac PA10 Analytical Column(4 mm×250 mm),the column Dionex CarboPac PA10 Guard Column(4 mm×50 mm)was used as the protective column.The mobile phase was NaOH and CH3 COONa,gradient elution procedure was used for separation and pulse integral amperometric for detection.Results The detection limits of 8 kinds of monosaccharides and disaccharides were 5,8,3,4,7,2,5,and 2μg/L,respectively,and all of them had a wide linear range of 0.05-20 mg/L.The relative standard deviation of disaccharide determination was 0.96%-6.12%,and the recoveries of 8 kinds of monosaccharides and disaccharides were 90.5%-102.1%.Conclusion The method can be used to detect monosaccharide and disaccharide in Aornial mealnocarpa fruit at same time.It is simple,rapid,effective,less matrix interference,derivative-free and highly sensitive,and is suitable for the analysis of common monosaccharides and disaccharides components in Aornial mealnocarpa fruit.

关 键 词：离子色谱法 黑果腺肋花楸果 单糖 双糖 

分 类 号：O657.7[理学—分析化学] TS207.3[理学—化学]